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Detection method details
Name: |
QL-TAX-GM-001 |
Description: |
Qualitative real-time PCR (SYBRGreen) method for detection of soybean lectin gene (Barbau-Piednoir et al., 2014) |
Comment: |
Target is the endogenous lectin (Le1) gene of Glycine max |
Validation: |
ring trial validation |
Standardisation: |
unknown |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Glycine max |
-
Oligonucleotides:
Forward Primer
Name:
sltm 1Sequence:
AACCGGTAGCGTTGCCAGSize:
18
Reverse Primer
Name:
sltm 2Sequence:
AGCCCATCTGCAAGCCTTTSize:
19
-
Amplicon:
Sequence:
aaccggtagcgttgccagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaaaggcttgcagatgggctSize:
81
Documents
Citation | Type | Local copy |
---|---|---|
Barbau-Piednoir, E., Stragier, P., Roosens, N., Mazzara, M., Savini, C., Van den Eede, G., Van den Bulcke, M. (2014). Inter-laboratory testing of GMO detection by combinatory SYBR®Green PCR screening (CoSYPS). Food Analytical Methods. 7, 1719-1728.![]() |
peer-reviewed article | |
EU-RL GMFF GMOMETHODS database; QL-TAX-GM-001![]() |
EU reference method protocol |