Detection method details
Name: |
QL-ELE-00-007 |
Description: |
Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998) |
Comment: |
Target is the nopaline synthase terminator (T-nos) from Agrobacterium tumefaciens |
Validation: |
ring trial validation |
Standardisation: |
national standard |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
T-nos-RHIRD
|
-
Oligonucleotides:
Forward Primer
Name:
NOS-1Sequence:
GAATCCTGTTGCCGGTCTTGSize:
20
Reverse Primer
Name:
NOS-3Sequence:
TTATCCTAGTTTGCGCGCTASize:
20
-
Amplicon:
Sequence:
gaatcctgttgccggtcttgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntagcgcgcaaactaggataaSize:
180
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QL-ELE-00-007 | EU reference method protocol |