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Detection Image Detection method details

Name:

 QT-TAX-GM-007

Description:

 Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene

Comment:

 target is the endogenous lectin (Le1) gene of soybean

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 taxon-specific

Target Species name:

 Glycine max

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Le1n02-5'

  • Sequence:

    GCCCTCTACTCCACCCCCA

  • Size:

    19

  • Reverse Primer

  • Name:

    Le1n02-3'

  • Sequence:

    GCCCATCTGCAAGCCTTTTT

  • Size:

    20

  • Probe

  • Name:

    Le1-Taq

  • Sequence:

    AGCTTCGCCGCTTCCTTCAACTTCAC

  • Size:

    26

  • Amplicon:

  • Sequence:

    gccctctactccacccccannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagcttcgccgcttccttcaacttcacnnnnnnnnnnnnnnnnnnaaaaaggcttgcagatgggc

  • Size:

    118

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-TAX-GM-007Link to the document url. EU reference method protocol
Shindo, Y., Kuribara, H., Matsuoka, T., Futo, S., Sawada, C., Shono, J., Akiyama, H., Goda, Y., Toyoda, M., Hino, A. (2002). Validation of real-time PCR analyses for line-specific quantitation of genetically modified maize and soybean using new reference molecules. Journal of the AOAC International, 85, 1119-1126Link to the document url. peer-reviewed article