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Detection Image Detection method details

Name:

 QT-TAX-GM-005

Description:

 Quantitative real-time PCR (TaqMan) for detection of soybean lectin gene (EU-RL GMFF GMOMETHODS database as of November 8, 2023).

Comment:

 Target is the endogenous lectin (Le1) gene of Glycine max

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 taxon-specific

Target Species name:

 Glycine max

  • Oligonucleotides:

  • Forward Primer

  • Name:

    lecF

  • Sequence:

    CCAGCTTCGCCGCTTCCTTC

  • Size:

    20

  • Reverse Primer

  • Name:

    lecR

  • Sequence:

    GAAGGCAAGCCCATCTGCAAGCC

  • Size:

    23

  • Probe

  • Name:

    lecP

  • Sequence:

    CTTCACCTTCTATGCCCCTGACAC

  • Size:

    24

  • Amplicon:

  • Sequence:

    ccagcttcgccgcttccttcnncttcaccttctatgcccctgacacnnnnnggcttgcagatgggcttgccttc

  • Size:

    74

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-TAX-GM-005Link to the document url. EU reference method protocol