Detection method details
Name: |
QT-EVE-ZM-005 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of maize event MON87460 (EU-RL GMFF GMOMETHODS database as of July 21, 2014) |
Comment: |
Target is the 5' integration border region (IBR) between the insert of maize event MON87460 and the maize host genome |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-ZM-002 |
Type: |
event-specific |
Target GMO name: |
MON87460 |
-
Oligonucleotides:
Forward Primer
Name:
MON 87460 primer 1Sequence:
CACGTTGAAGGAAAATGGATTGSize:
22
Reverse Primer
Name:
MON 87460 Primer 2Sequence:
TCGCGATCCTCCTCAAAGACSize:
20
Probe
Name:
MON 87460 ProbeSequence:
AGGGAGTATGTAGATAAATTTTCAAAGCGTTAGACGGCSize:
38
-
Amplicon:
Sequence:
cacgttgaaggaaaatggattgnagggagtatgtagataaattttcaaagcgttagacggcngtctttgaggaggatcgcgaSize:
82
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-005 | EU reference method protocol |