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Detection Image Detection method details

Name:

 QL-CON-00-001

Description:

 Qualitative conventional PCR method for detection of soybean event GTS 40-3-2 (ISO 21569)

Comment:

 Target is the junction region between the Cauliflower mosaic virus 35S promoter (CaMV P-35S) and the chloroplast transit peptide sequence from Petunia hybrida epsps gene (ctp4).

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

 QL-TAX-GM-002

Type:

 construct-specific

Target GMO names:
GTS 40-3-2

  • Oligonucleotides:

  • Forward Primer

  • Name:

    p35s-f2

  • Sequence:

    TGATGTGATATCTCCACTGACG

  • Size:

    22

  • Reverse Primer

  • Name:

    petu-r1

  • Sequence:

    TGTATCCCTTGAGCCATGTTGT

  • Size:

    22

  • Amplicon:

  • Sequence:

    tgatgtgatatctccactgacgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnacaacatggctcaagggataca

  • Size:

    172

Documents

Citation Type Local copy
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Qualitative nucleic acid based methods ISO 21569:1-69 (2005)Link to the document url. other document
EU-RL GMFF GMOMETHODS database; QL-CON-00-001Link to the document url. EU reference method protocol