Detection method details
Name: |
QL-ELE-00-004 |
Description: |
Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Lipp et al., 2001) |
Comment: |
Target is the Cauliflower mosaic virus 35S promoter (CaMV P-35S) |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
P-Cauliflower mosaic virus
|
-
Oligonucleotides:
Forward Primer
Name:
35S-cf3Sequence:
CCACGTCTTCAAAGCAAGTGGSize:
21
Reverse Primer
Name:
35S-cr4Sequence:
TCCTCTCCAAATGAAATGAACTTCCSize:
25
-
Amplicon:
Sequence:
ccacgtcttcaaagcaagtggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnggaagttcatttcatttggagaggaSize:
123