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Detection Image Detection method details

Name:

 QL-CON-00-015

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the bar gene (Bayer CropScience, 2006; verified by the EU-RL GMFF in the context of Commission Decision 2006/578/EC)

Comment:

 Target is the junction between the Cauliflower mosaic virus 35S promoter (CaMV P-35S) and the phosphinothricin N-acetyltransferase (bar) gene from Streptomyces hygroscopicus.

The fragment size generated by the QL-CON-00-015 PCR depends on the assembly of the two sequence elements and is varying in the different GM plant events.

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 construct-specific

Target GMO names:
Bt176, CBH351, DBT418, DLL25, EH-2 modbs 2.99, IE034, Iksan515, Iksan526, LLCotton25, LLRICE06, LLRICE601, LLRICE604, LLRICE62, MON88701, Ms3, Ms6, ND207, SYHT04R, T1c-19, T2A-1, T303-3, T304-40, TC6275, Varuna bn 3.6, Verdeca HB4, W62, W98

  • Oligonucleotides:

  • Forward Primer

  • Name:

    MDB498

  • Sequence:

    TATCCTTCGCAAGACCCTTCC

  • Size:

    21

  • Reverse Primer

  • Name:

    DPA143

  • Sequence:

    ATGTCGGCCGGGCGTCGTTCTG

  • Size:

    22

  • Probe

  • Name:

    TM099

  • Sequence:

    TCTATATAAGGAAGTTCATTTCATT

  • Size:

    25

  • Amplicon:

  • Sequence:

    tatccttcgcaagacccttcctctatataaggaagttcatttcatttggagaggacagggtaccggatccaacaatgagcccagaacgacgcccggccgacat

  • Size:

    103

Documents

Citation Type Local copy
Grain testing method for the detection of rice GM events containing 35S::bar sequences using RT-PCR protocols PGS0494 and PGS0476. (2006) Bayer CropScience. BioScience 31-8-2006, Ghent, BelgiumLink to the document url.
EU-RL GMFF GMOMETHODS database; QL-CON-00-015Link to the document url. EU reference method protocol