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Detection Image Detection method details

Name:

 QT-TAX-GM-004

Description:

 Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (Hird et al., 2003).

Comment:

 target is the endogenous lectin (Le1) gene of soybean

Validation:

 ring trial validation

Standardisation:

 unknown

Related Methods:

Type:

 taxon-specific

Target Species name:

 Glycine max

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Soya lectin-F

  • Sequence:

    TGGTCGCGCCCTCTACTC

  • Size:

    18

  • Reverse Primer

  • Name:

    Soya lectin-R

  • Sequence:

    GGCGAAGCTGGCAACG

  • Size:

    16

  • Probe

  • Name:

    Soya lectin

  • Sequence:

    CTACCGGTTTCTTTGTCCCAAATGTGGAT

  • Size:

    29

  • Amplicon:

  • Sequence:

    tggtcgcgccctctactcnnnnnnnatccacatttgggacaaagaaaccggtagcgttgccagcttcgcc

  • Size:

    70

Documents

Citation Type Local copy
Hird H., Powell J., Johnson M.L., Oehlschlager S.; Determination of percentage of RoundUp Ready soya in soya flour using real-time polymerase chain reaction: interlaboratory study" J AOAC Int 86:66-71 (2003)Link to the document url.
EU-RL GMFF GMOMETHODS database; QT-TAX-GM-004Link to the document url. EU reference method protocol