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Detection Image Detection method details

Name:

 QT-TAX-GM-009

Description:

 Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014)

Comment:

 Target is the endogenous lectin (Le1) gene from Glycine max

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 taxon-specific

Target Species name:

 Glycine max

  • Oligonucleotides:

  • Forward Primer

  • Name:

    KVM164

  • Sequence:

    CACCTTTCTCGCACCAATTGACA

  • Size:

    23

  • Reverse Primer

  • Name:

    KVM165

  • Sequence:

    TCAAACTCAACAGCGACGAC

  • Size:

    20

  • Probe

  • Name:

    TM021

  • Sequence:

    CCACAAACACATGCAGGTTATCTTGG

  • Size:

    26

  • Amplicon:

  • Sequence:

    cttctttctcgcaccaattgacannnnnccacaaacacatgcaggttatcttggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngtcgtcgctgttgagtttga

  • Size:

    105

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-TAX-GM-009Link to the document url. EU reference method protocol