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Detection Image Detection method details

Name:

 QL-CON-00-014

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the junction between the nopaline synthase promoter (P-nos)and the neomycin phosphotransferase II gene (nptII) (ISO/TS 21569-4)

Comment:

 target is the junction region of a construct of the nopaline synthase promoter(P-nos) from Agrobacterium tumefaciens and the neomycin phosphotransferase II (nptII) gene from Escherichia coli (amplicon in event Ms1 is 151 bp)

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 construct-specific

Target GMO names:
1345-4, 16-0-1, 18-2-4, 35 1 N, 55-1, 5B, 63-1, A1-DFR Petunias, B tomato, C5, Cantaloupe A, Cantaloupe B, Da tomato, EH92-527-1, F tomato, F3'5'H GM petunia, FP967, GD743, GFM Cry1A, GK19, GS784, H421, HCN10, HLMT15-15, HLMT15-3, HLMT15-46, Huafan No 1, Huanong No. 1, INI-FMAPDF, KMD1, MLS 9124, MPS965, Ms1, NF872, NXI-1T, NXI-4T, NXI-6T, PG451, RBMT15-101, RBMT21-129, RBMT21-152, RBMT21-350, RL01-15, RL01-17, RM3-3, RM3-4, RM3-6, Rf1, Rf2, SEMT15-02, SEMT15-15, SGK321, SY233, T120-7, TAM66274, Topas 19/2, X17-2

  • Oligonucleotides:

  • Forward Primer

  • Name:

    p-nos-F2

  • Sequence:

    TTCCCCTCGGTATCCAATTAGAG

  • Size:

    23

  • Reverse Primer

  • Name:

    NPTII-R

  • Sequence:

    GATTGTCTGTTGTGCCCAGTCA

  • Size:

    22

  • Probe

  • Name:

    NPTII-Tm2

  • Sequence:

    AGCCGAATAGCCTCTCCACCCAAGC

  • Size:

    25

  • Amplicon:

  • Sequence:

    ttcccctcggtatccaattagagtctcatattcactctcaatccagatccggcccatgatcatgtggattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatc

  • Size:

    151

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-CON-00-014Link to the document url. EU reference method protocol