Detection method details
Name: |
QT-TAX-ZM-014 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
Comment: |
Target is the endogenous alcohol deydrogenase 1 (adh1) gene from Zea mays. This assay is recommended for GMO quantitative analysis. |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Zea mays |
-
Oligonucleotides:
Forward Primer
Name:
Zm Adh1 primer FSequence:
CGTCGTTTCCCATCTCTTCCTCCSize:
23
Reverse Primer
Name:
Zm Adh1 primer RSequence:
CCACTCCGAGACCCTCAGTCSize:
20
Probe
Name:
Zm Adh1Sequence:
AATCAGGGCTCATTTTCTCGCTCCTCASize:
27
-
Amplicon:
Sequence:
cgtcgtttcccatctcttcctccnnnnnnnnnnnnnnnnnnnnaatcagggctcattttctcgctcctcannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngactgagggtctcggagtggSize:
135
Documents
Citation | Type | Local copy |
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