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Detection method details
Name: |
QT-TAX-GM-004 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (Hird et al., 2003). |
Comment: |
target is the endogenous lectin (Le1) gene of soybean |
Validation: |
ring trial validation |
Standardisation: |
unknown |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Glycine max |
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Oligonucleotides:
Forward Primer
Name:
Soya lectin-FSequence:
TGGTCGCGCCCTCTACTCSize:
18
Reverse Primer
Name:
Soya lectin-RSequence:
GGCGAAGCTGGCAACGSize:
16
Probe
Name:
Soya lectinSequence:
CTACCGGTTTCTTTGTCCCAAATGTGGATSize:
29
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Amplicon:
Sequence:
tggtcgcgccctctactcnnnnnnnatccacatttgggacaaagaaaccggtagcgttgccagcttcgccSize:
70