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Detection Image Detection method details

Name:

 QL-TAX-ZM-002

Description:

 Qualitative real-time PCR (SYBRGreen) method for detection of maize alcoholdeydrogenase 1 gene (Barbau-Piednoir et al., 2014)

Comment:

 Target is the endogenous alcohol dehydrogenase 1 (adh1) gene of Zea mays

Validation:

 ring trial validation

Standardisation:

 unknown

Related Methods:

Type:

 taxon-specific

Target Species name:

 Zea mays

  • Oligonucleotides:

  • Forward Primer

  • Name:

    ADH_alt Fwd

  • Sequence:

    TCTCTTCCTCCTTTAGAGCTACCACTA

  • Size:

    27

  • Reverse Primer

  • Name:

    ADH_alt Rev

  • Sequence:

    AATCGATCCAAAGCGAGATGA

  • Size:

    21

  • Amplicon:

  • Sequence:

    tctcttcctcctttagagctaccactannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntcatctcgctttggatcgatt

  • Size:

    83

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Citation Type Local copy