QL-CON-CP1/pFMV2 |
construct-specific |
GT200, GT73, GTSB77, H7-1, J101, J163, MON1445, MON1698, MON87705, MON88302, MON88913, MON89788, RBMT22-082, RBMT22-186, RBMT22-238, RBMT22-262, ZSR500, ZSR502, ZSR503 |
Qualitative conventional PCR method for detection of the junction between the FMV promoter and the cp4-epsps gene (BVL G30.40-11). |
ring trial validation |
national standard |
1 |
QL-CON-00-008 |
construct-specific |
2904/1 kgs, ASR368, DBN9004, DBN9936, GT200, GT73, GTSB77, H7-1, J101, J163, KWS20-1, MON1445, MON1698, MON71200, MON71700, MON71800, MON80100, MON802, MON809, MON832, MON87411, MON87427, MON87429, MON87705, MON88017, MON88302, MON88913, MON89788, NK603, RBMT22-082, RBMT22-186, RBMT22-238, RBMT22-262, ZSR500, ZSR502, ZSR503 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the chloroplast transit peptide 2 and the CP4 epsps gene (ISO 21569 AMD1). |
ring trial validation |
ISO standard |
1 |
QL-CON-00-013 |
construct-specific |
3006-210-23, 4114, DAS1507, DAS59122, DAS59132, KMD1, Kefeng6 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the maize ubiquitin promoter and the modified cry1Ab/1Ac genes (Grohmann et al.,2015). |
ring trial validation |
EU reference method |
1 |
QL-ELE-AgroBS1 F/AgroBS1 R |
element-specific |
V-Flanking Ti-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the nopaline type aka Agroborder I.
Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176) |
ring trial validation |
national standard |
1 |
QL-ELE-AgroBS2 F/AgroBS2 R |
element-specific |
V-Flanking Ti-ocs-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the octopine type aka Agroborder II.
Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176) |
ring trial validation |
national standard |
1 |
QL-ELE-AINT 2-5'/AINT 2-3' |
element-specific |
I-actin |
Qualitative real-time PCR (TaqMan) method for detection of rice actin 1 intron (I-ractI) (Mano et al. 2009). |
in-house validation |
none |
1 |
QL-ELE-epsps 1-5'/epsps 3-3' |
element-specific |
CS-CP4epsps-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the CP4-epsps gene (Scholtens et al. 2013). |
in-house validation |
none |
1 |
QL-ELE-epsps2 1-5'/epsps2 1-3' |
element-specific |
CS-CP4epsps-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the CP4 epsps gene (Mano et al., 2009) |
in-house validation |
none |
1 |
QL-ELE-Patf-n2/Patr-n2 |
element-specific |
CS-pat-STRVR |
Qualitative real-time PCR (TaqMan) method for detection of the pat gene (Xu et al., 2006) |
in-house validation |
none |
1 |
QL-ELE-P-Ubi-F/P-Ubi-R |
element-specific |
P-ubi1-MAIZE |
Qualitative real-time PCR (TaqMan) method for detection of ubiquitin promoter of Zea mays (Debode et al., 2013) |
in-house validation |
none |
1 |
QL-ELE-00-006 |
element-specific |
T-nos-RHIRD |
Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31) |
ring trial validation |
national standard |
1 |
QL-ELE-00-007 |
element-specific |
T-nos-RHIRD |
Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998) |
ring trial validation |
national standard |
1 |
QL-ELE-00-009 |
element-specific |
T-nos-RHIRD |
Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-010 |
element-specific |
E-FMV, P-34S FMV |
Qualitative conventional PCR method for detection of Figwort mosaic virus 34S promoter (Pan et al., 2007) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-011 |
element-specific |
T-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1). |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-012 |
element-specific |
E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH |
Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S).
Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-013 |
element-specific |
T-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos).
Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-015 |
element-specific |
E-FMV, P-34S FMV |
Qualitative real-time PCR (TaqMan) method for detection of Figwort mosaic virus 34S promoter (ISO/TS 21569-5). |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-018 |
element-specific |
T-nos-RHIRD |
Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014) |
ring trial validation |
unknown |
1 |
QL-ELE-00-019 |
element-specific |
CS-CP4epsps-RHIRD |
Qualitative real-time PCR (SYBRGreen) method for detection of CS-CP4epsps-RHIRD (Barbau-Piednoir et al., 2014) |
ring trial validation |
unknown |
1 |
QL-ELE-00-021 |
element-specific |
CS-pat-STRVR |
Qualitative real-time PCR (SYBRGreen) method for detection of CS-pat-STRVR (Barbau-Piednoir et al., 2014) |
ring trial validation |
unknown |
1 |
QL-ELE-00-024 |
element-specific |
T-rbcS_E9-PEA |
Qualitative real-time PCR (TaqMan) method for detection of the terminator of the pea ribulose-1,5-bisphosphate carboxylase small subunit (rbcS) E9 gene (EU-RL GMFF GMOMETHODS database as of January 20, 2017). |
ring trial validation |
EU reference method |
1 |
QL-ELE-00-025 |
element-specific |
CS-pat-STRVR |
Qualitative real-time PCR (TaqMan) method for detection of the synthetic phosphinothricin acetyltransferase (pat) gene (BVL L00.00-154). EU-RL GMFF GMOMETHODS database as of December 23, 2016. |
ring trial validation |
EU reference method |
1 |
QT-ELE-NOS ter 2-5' /NOS ter 2-3' |
element-specific |
T-nos-RHIRD |
Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009) |
ring trial validation |
none |
1 |
QT-ELE-00-002 |
element-specific |
CS-pat-STRVR |
Quantitative real-time PCR (TaqMan) method for detection of phosphinothricin N-acetyltransferase gene (Weighardt et al., 2004) (EURL GMFF GMOMETHODS database as of February 5, 2020) |
ring trial validation |
unknown |
1 |
QT-ELE-00-004 |
element-specific |
E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH |
Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1) |
ring trial validation |
ISO standard |
1 |
QT-EVE-GH-001a |
event-specific |
281-24-236 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event 281-24-236 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) |
ring trial validation |
EU reference method |
1 |
QT-EVE-GH-001b |
event-specific |
3006-210-23 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event 3006-210-23 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) |
ring trial validation |
EU reference method |
1 |
QT-EVE-GH-007 |
event-specific |
MON88913 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event MON88913 (EU-RL GMFF GMOMETHODS database as January 13, 2014) |
ring trial validation |
EU reference method |
1 |
QT-EVE-GH-012 |
event-specific |
COT102 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event COT102 (EU-RL GMFF GMOMETHODS database as of February 18, 2020) |
ring trial validation |
EU reference method |
1 |
QT-TAX-GH-015 |
taxon-specific |
Gossypium hirsutum |
Quantitative real-time PCR (TaqMan) method for detection of cotton fiber-specific acyl carrier protein (ACP1) gene (EU-RL GMFF GMOMETHODS database as of January 07, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
ring trial validation |
EU reference method |
1 |
QT-TAX-GH-016 |
taxon-specific |
Gossypium hirsutum |
Quantitative PCR method for detection of putative cotton SAH7 protein gene (123 bp) (Mazzara et al., 2006).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
ring trial validation |
EU reference method |
1 |
QT-TAX-GH-018 |
taxon-specific |
Gossypium hirsutum |
Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of January 17, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation |
EU reference method |
1 |
QT-TAX-GH-019 |
taxon-specific |
Gossypium hirsutum |
Quantitative real-time PCR (Taqman) method for detection of cotton alcohol dehydrogenase C gene (EU-RL GMFF GMOMETHODS database as of November 1, 2018).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation |
EU reference method |
1 |
QT-TAX-GH-020 |
taxon-specific |
Gossypium hirsutum |
Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of July 2020).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation |
EU reference method |
1 |
QT-TAX-GH-021 |
taxon-specific |
Gossypium hirsutum |
Quantitative real-time PCR (TaqMan) method for detection of putative cotton SAH7 protein gene (115bp) (EU-RL GMFF GMOMETHODS database as January 22, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
ring trial validation |
EU reference method |
1 |