Description:
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The cry1Ac, nptII and aad genes were introduced into cotton Coker 312 on a plasmid vector PV-GHBK04 carried by Agrobacterium tumefaciens.
Southern blot analysis was used to demonstrate that two T-DNA copies inserted in a head-to-tail arrangement were present in the genome of INGARD cotton. One T-DNA copy contains full-length copies of the cry1Ac, nptII and aad genes, the second is a partial copy, containing only a portion of the cry1Ac gene that does not encode the insecticidally active region of the Bt protein.
Additionally, a non-functional fragment containing the right border T-DNA sequence and a portion of the T-7Salpha-SOYBN was separately inserted.
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Links regarding to this GMO:
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OGTR http://content.webarchive.nla.gov.au/gov/wayback/20080812063909/http://www.health.gov.au/internet/ogtr/publishing.nsf/Content/dir008-3/$FILE/dir008finalrarmp.pdf,
ABCA http://www.abca.com.au/wp-content/uploads/2012/09/ABCA_Resource_Guide_3_v2.pdf,
BCH http://bch.cbd.int/database/record.shtml?documentid=14775,
ISAAA http://www.isaaa.org/gmapprovaldatabase/event/default.asp?EventID=54,
EFSA Scientific Opinion https://efsa.onlinelibrary.wiley.com/doi/epdf/10.2903/j.efsa.2011.2373
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Transformation / Modification technique:
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Agrobacterium-induced
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