GMO molecular characterization details

GMO detailed information

GMO:
MON810
Alias:
UID:
MON-ØØ81Ø-6
Tradename:

YieldGard Corn Borer
Company:
Bayer Crop Science (formerly Monsanto Company)
Developer:

Bayer Crop Science (formerly Monsanto Company)
Species:
Zea mays
(maize, corn)
Traits:
EU authorisation:

Inserts / Modification

Name:	insert_MON810
Comment:	Entered according to BCH Record ID 14750
Map:

Genetic Elements:

Reference: BCH

Elements:

Order	Name	Donor	Position	Definition	Trait	Comment
1	P-e35S-CaMV	Cauliflower mosaic virus	-	Cauliflower mosaic virus (CaMV) 35S promoter with duplicated enhancer region; inserted element length can vary between e.g. 307bp/322bp/296bp/551bp/615bp; Kay R, et al. (1987) Science 236, 1299-1302
2	I-hsp70-MAIZE	Zea mays	-
3	CS-cry1Ab10-BACTK	Bacillus thuringiensis ssp. Kurstaki	-	plant optimized gene coding for the nature identical (full length) cry1Ab gene of B. thuringiensis ssp. Kurstaki HD-1 strain; Fischhoff D et al, (1987). Bio/Technology, 5, 807-813	Lepidoptera resistance	truncated version of CS-cry1Ab-BACTU

Related DNA Sequences:

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	BCH	No
JQ406878	NCBI - GenBank	Yes	855	Zea mays transgenic MON810 3' insertion site genomic sequence
JQ406879	NCBI - GenBank	Yes	1018	JQ406879\| Zea mays transgenic MON810 5' insertion site genomic sequence
RIKILT20151130	Wageningen Food Safety Research (WFSR)	Yes	5122	Merged MON810 sequence of JQ406879 and AY326434
RIKILT_MON810_event	Wageningen Food Safety Research (WFSR)	Yes	92	Amplicon sequence of MON810 for event-specific method QT-EVE-ZM-020 without probe
RIKILT_P-35S_element_MON810	Wageningen Food Safety Research (WFSR)	Yes	101	Amplicon sequence of P-35S for element-specific method QT-ELE-P35S 1-5'/P35S 1-3' without probe
RIKILT_cry1ab_element_MON810	Wageningen Food Safety Research (WFSR)	Yes	152	Amplicon sequence of cry1Ab for element-specific method QL-ELE-cry1A 4-5'/cry1A 4-3' without probe
RIKILT_hsp70-cry1Ab_construct_MON810	Wageningen Food Safety Research (WFSR)	Yes	113	Amplicon sequence of hsp70-cry1Ab for construct-specific method QT-CON-00-004 without probe

Detection Methods

23 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-ELE-cry1A 4-5'/cry1A 4-3'	element-specific	CS-cry1Ab-BACTU	Qualitative real-time PCR (TaqMan) method for detection of the cry1Ab gene (Scholtens et al. 2013).	in-house validation	none	3
QL-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	2
QL-ELE-00-004	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Lipp et al., 2001)	ring trial validation	ISO standard	2
QL-ELE-00-005	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (ISO/FDIS 21569)	ring trial validation	ISO standard	2
QL-ELE-00-012	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1)	ring trial validation	ISO standard	2
QL-ELE-00-017	element-specific	P-Cauliflower mosaic virus	Qualitative real-time PCR (SYBRGreen) method for detection of P-35S CaMV (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	2
QL-ELE-00-020	element-specific	CS-cry1Ab-BACTU	Qualitative real-time PCR (SYBRGreen) method for detection of CS-cry1Ab-BACTU (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-EVE-ZM-001	event-specific	MON810	Qualitative conventional PCR method for the detection of maize event MON810 (ISO/FDIS 21569).	ring trial validation	ISO standard	3
QL-TAX-ZM-002	taxon-specific	Zea mays	Qualitative real-time PCR (SYBRGreen) method for detection of maize alcoholdeydrogenase 1 gene (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-TAX-ZM-003	taxon-specific	Zea mays	Qualitative conventional PCR method for detection of the maize invertase gene (ISO 21569)	ring trial validation	ISO standard	1
QT-CON-00-004	construct-specific	DBN9936, MON80100, MON802, MON809, MON810	Quantitative real-time PCR (TaqMan) method for detection of maize event MON810 (Shindo et al., 2002)	ring trial validation	ISO standard	3
QT-ELE-P35S 1-5'/P35S 1-3'	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of the Cauliflower mosaic virus 35S promoter (Kodama et al., 2009)	ring trial validation	national standard	3
QT-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (Feinberg et al., 2005)	ring trial validation	unknown	2
QT-ELE-00-004	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1)	ring trial validation	ISO standard	3
QT-EVE-ZM-020	event-specific	MON810	Quantitative real-time PCR (TaqMan) method for detection of maize event MON810 (ISO/FDIS 21570)	ring trial validation	ISO standard	3
QT-TAX-ZM-001	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene. Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	ISO standard	1
QT-TAX-ZM-002	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of the maize high-mobility-group A (hmgA) gene (ISO 21570). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	ISO standard	1
QT-TAX-ZM-003	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene (EU-RL GMFF GMOMETHODS database as of October 01, 2014)	ring trial validation	EU reference method	1
QT-TAX-ZM-004	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) for detection of maize aldolase 1 (ald1) gene (EU-RL GMFF GMOMETHODS database as of July 14, 2016). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1
QT-TAX-ZM-005	taxon-specific	Zea mays	Quantitative PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of May 28, 2018).	ring trial validation	EU reference method	1
QT-TAX-ZM-006	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (ISO 21570)	ring trial validation	ISO standard	1
QT-TAX-ZM-011	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of the maize alcohol dehydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014)	ring trial validation	EU reference method	1
QT-TAX-ZM-014	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1

Reference Materials

Catalogue number	Source	Value	Type	Certified
ERM-AD413	JRC - CRM (ERM)	2·10E06 cp/µl	plasmid DNA	yes
ERM-BF413ak	JRC - CRM (ERM)	<0.09 % (m/m)	dried powder	yes
ERM-BF413ck	JRC - CRM (ERM)	0.49 % (m/m)	dried powder	yes
ERM-BF413ek	JRC - CRM (ERM)	1.98 % (m/m)	dried powder	yes
ERM-BF413gk	JRC - CRM (ERM)	9.9 % (m/m)	dried powder	yes
5211500901	Eurofins GeneScan	100 cp/µl	genomic DNA	no
5211513902	Eurofins GeneScan	20 cp /µl	genomic DNA	no
5211513920	Eurofins GeneScan	20 cp /µl	genomic DNA	no

Authorisation

Your Search criteria is: ( GMO: MON810 ) AND ( Species: Zea mays )

Refine Search 25 results

Continent	Country	File Reference	Intendend Use Details	Legal Status Details	Legislation	Decision Date
Europe	EU	GMFF-2022-9450 EFSA-GMO-RX-028	food - food and food ingredients produced from GMO	not approved - pending	Regulation (EC) No 1829/2003
Europe	EU	GMFF-2022-9450 EFSA-GMO-RX-028	other uses - products other than food and feed containing or consisting of GMO for the same uses as any other of this species with the exception of cultivation	not approved - pending	Regulation (EC) No 1829/2003
Europe	EU	GMFF-2022-9450 EFSA-GMO-RX-028	feed - feed containing, consisting of, or produced from GMO	not approved - pending	Regulation (EC) No 1829/2003
Europe	EU	GMFF-2022-9450 EFSA-GMO-RX-028	cultivation - cultivation	not approved - not submitted	Regulation (EC) No 1829/2003
Europe	EU	GMFF-2022-9450 EFSA-GMO-RX-028	food - pollen produced from this GMO, as or in foods and food ingredients	not approved - pending	Regulation (EC) No 1829/2003
Europe	EU	EFSA-GMO-RX-MON810 (8.1.b-20.1.b)	other uses - products other than food and feed containing or consisting of GMO for the same uses as any other of this species with the exception of cultivation	not approved - not submitted	Regulation (EC) No 1829/2003	July 4, 2017
Europe	EU	EFSA-GMO-RX-MON810 (20.1.a)	feed - feed containing or consisting of GMO	approved - approved	Regulation (EC) No 1829/2003	July 4, 2017
Europe	EU	EFSA-GMO-RX-MON810 (20.1.a)	food - foods and food ingredients containing, consisting of, or produced from GMO	not approved - not submitted	Regulation (EC) No 1829/2003	July 4, 2017
Europe	EU	EFSA-GMO-RX-MON810 (20.1.a)	cultivation - cultivation	not approved - pending	Regulation (EC) No 1829/2003	July 4, 2017
Europe	EU	EFSA-GMO-RX-MON810 (20.1.a)	other uses - products other than food and feed containing or consisting of GMO for the same uses as any other of this species with the exception of cultivation	approved - approved	Regulation (EC) No 1829/2003	July 4, 2017
Europe	EU	EFSA-GMO-RX-MON810 (8.1.b-20.1.b)	cultivation - cultivation	not approved - not submitted	Regulation (EC) No 1829/2003	July 4, 2017
Europe	EU	EFSA-GMO-RX-MON810 (8.1.b-20.1.b)	food - food additives produced from GMO	approved - approved	Regulation (EC) No 1829/2003	July 4, 2017
Europe	EU	EFSA-GMO-RX-MON810 (8.1.b-20.1.b)	feed - feed produced from GMO (feed materials and feed additives)	approved - approved	Regulation (EC) No 1829/2003	July 4, 2017
Europe	EU	EFSA-GMO-RX-MON810 (8.1.a)	food - food and food ingredients produced from GMO	approved - approved	Regulation (EC) No 1829/2003	July 4, 2017
Europe	EU	EFSA-GMO-RX-MON810 (8.1.a)	other uses - products other than food and feed containing or consisting of GMO for the same uses as any other of this species with the exception of cultivation	not approved - not submitted	Regulation (EC) No 1829/2003	July 4, 2017
Europe	EU	EFSA-GMO-RX-MON810 (8.1.a)	feed - feed containing, consisting of, or produced from GMO	not approved - not submitted	Regulation (EC) No 1829/2003	July 4, 2017
Europe	EU	EFSA-GMO-RX-MON810 (8.1.a)	cultivation - cultivation	not approved - not submitted	Regulation (EC) No 1829/2003	July 4, 2017
Europe	EU	EFSA-GMO-NL-2012-107	other uses - products other than food and feed containing or consisting of GMO for the same uses as any other of this species with the exception of cultivation	not approved - not submitted	Regulation (EC) No 1829/2003	November 6, 2013
Europe	EU	EFSA-GMO-NL-2012-107	feed - feed containing, consisting of, or produced from GMO	not approved - not submitted	Regulation (EC) No 1829/2003	November 6, 2013
Europe	EU	EFSA-GMO-NL-2012-107	cultivation - cultivation	not approved - not submitted	Regulation (EC) No 1829/2003	November 6, 2013
Europe	EU	EFSA-GMO-NL-2012-107	food - pollen produced from this GMO, as or in foods and food ingredients	approved - approved	Regulation (EC) No 1829/2003	November 6, 2013
Europe	EU	C/F/95/12-02	cultivation - cultivation	approved - approved	Directive 90/220/EEC	August 3, 1998
Europe	EU	C/F/95/12-02	food - foods and food ingredients containing, consisting of, or produced from GMO	not approved - not approved	Directive 90/220/EEC	August 3, 1998
Europe	EU	C/F/95/12-02	feed - feed containing or consisting of GMO	approved - approved	Directive 90/220/EEC	August 3, 1998
Europe	EU	C/F/95/12-02	other uses - products other than food and feed containing or consisting of GMO for the same uses as any other of this species	approved - approved	Directive 90/220/EEC	August 3, 1998

Description:
Links regarding to this GMO:
Transformation / Modification technique:	biolistic method

Citation	Publication year	Publishing authority	Publication type	Content types
Application for renewal of the authorisations to place on the market MON 810 maize under Regulation (EC) No 1829/2003 on genetically modified food and feed (Commission Implementing Decision 2013/649/EU of 6 November 2013) and (Commission Implementing Decision 2017/1207/EU of 4 July 2017)	2023	EFSA	application summary	application summary
Commission implementing decision (EU) 2017/1207 of 4 July 2017 renewing the authorisation for the placing on the market of genetically modified maize MON810 (MON-ØØ81Ø-6) products pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council (notified under document C(2017) 4453)	2017	EU	EU Commission Decision	decision document
COMMISSION IMPLEMENTING DECISION of 6 November 2013 authorising the placing on the market of pollen produced from maize MON 810 (MON-ØØ81Ø-6) pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council (notified under document C(2013)	2013	EU	EU Commission Decision	decision document
Scientific Opinion on an application (EFSA-GMO-NL-2012-107) for the placing on the market of maize MON 810 pollen under Regulation (EC) No 1829/2003 from Monsanto	2012	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Application for authorization to place on the market MON 810 pollen in the European Union, according to Regulation (EC) No 1829/2003 on genetically modified food and feed. Part VII Summary	2012	EFSA	application summary	application summary
Overall opinion of the European Food Safety Authority in accordance with Article 6 of Regulation (EC) No 1829/2003 on application (reference EFSA-GMO-NL-2012-107) for the placing on the market of food produced from or containing ingredients produced from maize MON 810, according to Article 3(1)(c) of Regulation (EC) No 1829/2003 (maize MON 810 pollen as or in food) (application EFSA-GMO-NL-2012-107) from Monsanto	2012	EFSA	EFSA overall opinion	risk assessment document
Overall opinion of the European Food Safety Authority in accordance with Article 18 of Regulation (EC) No 1829/2003 on application EFSA-GMO-RX-MON810[20-1a] for renewal of the authorisation of existing products produced from insect resistant genetically modified maize MON810[20-1a], and seeds for cultivation under Regulation (EC) No 1829/2003 from Monsanto	2009	EFSA	EFSA overall opinion	risk assessment document
Application for renewal of the authorisation for continued marketing of existing MON810 maize products that were authorized under Directive 90/220/EEC (Decision 98/294/EC) and subsequently notified in accordance to Article 20(1)(a) of Regulation (EC) No 1829/2003 on genetically modified food and feed. Part II - Summary	2009	EFSA	application summary	application summary
Application for renewal of the authorisation for continued marketing of existing food and food ingredients produced from MON810 maize that were notified pursuant to Article 5 of Regulation (EC) No 258/97 and subsequently notified according to Article 8(1)(a) of Regulation (EC) No 1829/2003 on genetically modified food and feed. Part II - Summary	2009	EFSA	application summary	application summary
Overall opinion of the European Food Safety Authority in accordance with Articles 6 and 18 of Regulation (EC) No 1829/2003 on application EFSA-GMO-RX-MON810[8-1b/20-1b] for renewal of the authorisation of existing products produced from insect resistant genetically modified maize MON810[8-1b/20-1b], under Regulation (EC) No 1829/2003 from Monsanto	2009	EFSA	EFSA overall opinion	risk assessment document
Application for renewal of the authorisation for continued marketing of existing food additives, feed materials and feed additives produced from MON810 maize that were notified according to Articles 8(1)(b) and 20(1)(b) of Regulation (EC) No 1829/2003 on genetically modified food and feed. Part II - Summary	2009	EFSA	application summary	application summary
Scientific Opinion - Applications (EFSA-GMO-RX-MON810) for renewal of authorisation for the continued marketing of (1) existing food and food ingredients produced from genetically modified insect resistant maize MON810; (2) feed consisting of and/or containing maize MON810, including the use of seed for cultivation; and of (3) food and feed additives, and feed materials produced from maize MON810, all under Regulation (EC) No 1829/2003 from Monsanto	2009	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Scientific Opinion of GMO Panel: Applications (EFSA-GMO-RX-MON810) for renewal of authorisation for the continued marketing of (1) existing food and food ingredients produced from genetically modified insect resistant maize MON810; (2) feed consisting of and/or containing maize MON810, including the use of seed for cultivation; and of (3) food and feed additives, and feed materials produced from maize MON810, all under Regulation (EC) No 1829/2003 from Monsanto	2009	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Grisolia, C. K., Oliveira, R., Domingues, I., Oliveira-Filho, E. C., Monerat, R. G., & Soares, A. M. V. M. (2009). Genotoxic evaluation of different delta-endotoxins from Bacillus thuringiensis on zebrafish adults and development in early life stages. Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 672(2), 119-123.	2009	Research group	peer-reviewed article	safety
Overall opinion of the European Food Safety Authority in accordance with Article 6 of Regulation (EC) No 1829/2003 on application EFSA-GMO-RX-MON810[8-1a] for renewal of the authorisation of existing products produced from insect resistant genetically modified maize MON810[8-1a], under Regulation (EC) No 1829/2003 from Monsanto.	2009	EFSA	EFSA overall opinion	risk assessment document
Commission decision of 22 April 1998 concerning the placing on the market of genetically modified maize (Zea mays L. line MON 810), pursuant to Council Directive 90/220/EEC (98/294/EC)	1998	EU	EU Commission Decision	decision document
Commission Decision of 22 April 1998 concerning the placing on the market of genetically modified maize (Zea mays L. line MON 810), pursuant to Council Directive 90/220/EEC. (98/294/EC) Official Journal of the European Communities 5. 5. 98	1998	EU	EU Commission Decision	decision document

European GMO Initiative for a Unified Database System

GMO detailed information

GMO:

Alias:

UID:

Tradename:

Company:

Developer:

Species:

Traits:

EU authorisation:

General Information

Links regarding to this GMO:

Inserts / Modification

Transformation Vectors

Detection Methods

Reference Materials

Authorisation

Literature reference