1 |
V-LB-RHIRD |
Agrobacterium tumefaciens |
|
- |
|
|
derived from Ti plasmid pTiT37
|
2 |
V-intervening-SYNTH |
Synthetic |
|
- |
|
|
length: 562 bp, comprises a 406 bp-long fragment of the origin of replication (ori) from bacteriophage M13 and a 156 bp long fragment of CS-beta_gal-ECOLX of Escherichia coli strain ECO which is a derivative of E. coli K12 |
3 |
TP-nls-SYNTH |
Synthetic |
|
- |
synthetic nuclear localisation sequence (NLS). |
|
length: 529 bp |
4 |
CS-pg_RNAi_as-SOLLC |
Solanum lycopersicum |
|
- |
Fragment of the polygalacturonase (pg) gene from Lycopersicon esculentum var. Ailsa Craig in antisense orientation. Grierson et al. (1986). Nucleic Acids Res, 14, 8595-8605; Smith et al. (1988). Nature, 334, 724-726 |
Altered ripening or flowering |
length: 731 bp; first 731 bp of a cDNA clone in antisense orientation that was derived from the native CS-pg-SOLLC of Solanum lycopersicum (Lycopersicon esculentum Mill. variety Ailsa Craig) |
5 |
T-nos-RHIRD |
Agrobacterium tumefaciens |
|
- |
3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807 |
|
length: 247 bp |
6 |
V-intervening-SYNTH |
Synthetic |
|
- |
|
|
length: 671 bp; comprises a 231 bp-long fragment of CS-beta_gal-ECOLX of Escherichia coli strain ECO which is a derivative of E. coli K12 and a 440 bp long internal fragment of Gene III from bacteriophage M13 which is a component of the viral coat |
7 |
T-nos-RHIRD |
Agrobacterium tumefaciens |
|
- |
3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807 |
|
length: 258 bp |
8 |
V-intervening-SYNTH |
Synthetic |
|
- |
|
|
length: 209 bp, internal fragment of ornithine cyclodeaminase gene (CS-ocd-RHIRD) and fragment of unspecified vector backbone |
9 |
CS-nptII-ECOLX |
Escherichia coli |
|
- |
Neomycin phosphotransferase II gene from the Eschrichia coli Tn5 transposon. Beck et al (1982). Gene, 19, 327-336 |
Kanamycin/neomycin resistance |
length: 800 bp |
10 |
P-nos-RHIRD |
Agrobacterium tumefaciens |
|
- |
Promoter region of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983). Nucleic Acids Res 11, 369-385; Fraley et al. (1983). Proc Nat L Acad Sci 80, 4803-4807 |
|
length: 227 bp |
11 |
V-RB-RHIRD |
Agrobacterium tumefaciens |
|
- |
|
|
derived from Ti plasmid pTiT37 |