Description:
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MON87419 was produced by Agrobacterium-mediated transformation of maize tissue using the 2 T-DNA transformation plasmid vector PV-ZMHT507801. This plasmid vector contains two separate T-DNAs.
The first T-DNA contains
- the dmo expression cassette and,
- the pat expression cassette.
The second T-DNA contains:
- the cp4-epsps expression cassette for selection.
During transformation, both T-DNAs were inserted into the maize genome. Subsequently, traditional breeding, segregation, selection and screening were used to isolate plants that contained the dmo expression cassette and the pat expression cassette and did not contain the cp4-epsps expression cassette.
Molecular characterization of the DNA insert demonstrated that MON87419 contains one copy of the intended transfer DNA containing the dmo expression cassette and the pat expression cassette that is stably inherited over multiple generations and segregates according to Mendelian principles.
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Links regarding to this GMO:
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USDA-APHIS http://www.aphis.usda.gov/brs/aphisdocs/15_11301p.pdf,
BCH https://bch.cbd.int/en/database/LMO/BCH-LMO-SCBD-111531-4
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Transformation / Modification technique:
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Agrobacterium-induced
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