Description:
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AM04-1020 was developed through Agrobacterium-mediated transformation of the potato variety Kuras.
The intended consequence of the genetic modification in AM04-1020 is to inhibit the expression of granule bound starch synthase protein (GBSS) responsible for amylose biosynthesis. As a result, the starch produced has little or no amylose and consists of amylopectin, which modifies the physical properties of the starch.
The csr1-2 gene encoding acetohydroxyacid synthase (AHAS) from Arabidopsis thaliana was included in the transformation process as marker conferring tolerance to imidazolinone herbicides.
The T-DNA was integrated at a single locus and as a single copy in the potato nuclear genome.
AM04-1020 was confirmed to be free of Agrobacterium-derived vector backbone DNA. Molecular analysis indicated that the DNA insert was stable over multiple generations of vegetatively propagated material.
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Links regarding to this GMO:
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FDA-Biotechnology Consultation http://www.accessdata.fda.gov/scripts/fdcc/?set=Biocon&id=BPS-A-1020-5,
BCH https://bch.cbd.int/en/database/record?documentID=48075
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Transformation / Modification technique:
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Agrobacterium-induced
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