Description:
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The self-limiting GE Aedes aegypti (mosquito) line OX5034 expresses (1) a conditional female lethality trait and (2) a fluorescent marker.
Repressible female specific lethality is achieved using alternative splicing and the expression of a synthetic tetracycline‐transcriptional activator (tTAV) variant. In the environment, without the presence of tetracycline, female offspring of the OX5034 mosquitoes are expected to die before they mature into adults.
Due to the alternative splicing mechanism, males carry and transmit the self-limiting genes to progeny without requiring tetracycline. Population suppression is thus achieved through the biased selection of male offspring.
The expressed fluorescent marker DsRed2, a synthetically modified variant of the original red fluorescent protein isolated from Discosoma sp., aids in the detection of Aedes aegypti carrying the OX5034 rDNA construct. A synthetic nuclear localisation signal was included to the expression cassette to localise DsRed2 to the nuclei of cells.
In comparison to line OX513, the second generation self-limiting mosquito line OX5034 has additional features: the genetic sex-separation enables more targeted production and release of only male mosquitoes while the additional nuclear localisation signal (nls) improves the visualisation of the fluorescence which enables field monitoring in all life stages of the mosquito.
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Links regarding to this GMO:
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BCH https://bch.cbd.int/database/record.shtml?documentid=115346
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Transformation / Modification technique:
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microinjection
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