GMO molecular characterization details

GMO detailed information

GMO:
QCAV-4
Alias:
UID:
QUT-QCAV4-6
Tradename:
Companies/Institutions:
Queensland University of Technology (QUT - Brisbane, Australia)
Species:
Musa acuminata
(Banana)
Traits:
EU authorisation:

no Reference materials could be found
No results found.
no Literature reference could be found

General Information

Inserts / Modification

Name:	insert_QCAV-4
Comment:	A large T-DNA insertion of 26,849 bp is located on banana chromosome 6. The insert contains three full, intact and functional copies of the 6,702 bp T-DNA as well as two fragmented portions of the RGA2 expression cassette recombined in opposite directions and inserted between T-DNA2 and T-DNA3.
Map:

Genetic Elements:

Reference: FSANZ

Elements:

Order	Name	Donor	Position	Definition	Trait	Comment
1	V-LB-RHIRD	Agrobacterium tumefaciens	-
2	T-35S-CaMV	Cauliflower mosaic virus	-	3' untranslated region (3' UTR) of the 35S gene from Cauliflower mosaic virus. Gardner et al. (1981). Nucleic Acids Res, 9:2871-2888
3	CS-nptII-ECOLX	Escherichia coli	-	Neomycin phosphotransferase II gene from the Eschrichia coli Tn5 transposon. Beck et al (1982). Gene, 19, 327-336	Kanamycin/neomycin resistance
4	P-35S-CaMV	Cauliflower mosaic virus	-
5	P-nos-RHIRD	Agrobacterium tumefaciens	-	Promoter region of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983). Nucleic Acids Res 11, 369-385; Fraley et al. (1983). Proc Nat L Acad Sci 80, 4803-4807
6	CS-rga2-MUSAC	Musa acuminata	-	Gene from Musa acuminata conferring resistance to Fusarium wilt tropical race 4. GenBank EU616673/ACF21694. Peraza-Echeverria et al. (2009). Mol Breed. 23:431-443 doi: 10.1007/s11032-008-9247-6	Fusarium wilt tropical race 4 resistance
7	T-nos-RHIRD	Agrobacterium tumefaciens	-	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807
8	V-RB-RHIRD	Agrobacterium tumefaciens	-
9	V-RB-RHIRD	Agrobacterium tumefaciens	-
10	T-nos-RHIRD	Agrobacterium tumefaciens	-	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807
11	CS-rga2-MUSAC	Musa acuminata	-	Gene from Musa acuminata conferring resistance to Fusarium wilt tropical race 4. GenBank EU616673/ACF21694. Peraza-Echeverria et al. (2009). Mol Breed. 23:431-443 doi: 10.1007/s11032-008-9247-6	Fusarium wilt tropical race 4 resistance
12	P-nos-RHIRD	Agrobacterium tumefaciens	-	Promoter region of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983). Nucleic Acids Res 11, 369-385; Fraley et al. (1983). Proc Nat L Acad Sci 80, 4803-4807
13	P-35S-CaMV	Cauliflower mosaic virus	-
14	CS-nptII-ECOLX	Escherichia coli	-	Neomycin phosphotransferase II gene from the Eschrichia coli Tn5 transposon. Beck et al (1982). Gene, 19, 327-336	Kanamycin/neomycin resistance
15	T-35S-CaMV	Cauliflower mosaic virus	-	3' untranslated region (3' UTR) of the 35S gene from Cauliflower mosaic virus. Gardner et al. (1981). Nucleic Acids Res, 9:2871-2888
16	V-LB-RHIRD	Agrobacterium tumefaciens	-
17	CS-rga2-MUSAC	Musa acuminata	-	Gene from Musa acuminata conferring resistance to Fusarium wilt tropical race 4. GenBank EU616673/ACF21694. Peraza-Echeverria et al. (2009). Mol Breed. 23:431-443 doi: 10.1007/s11032-008-9247-6	Fusarium wilt tropical race 4 resistance	3,042 bp antisense portion of the RGA2 ORF
18	CS-rga2-MUSAC	Musa acuminata	-	Gene from Musa acuminata conferring resistance to Fusarium wilt tropical race 4. GenBank EU616673/ACF21694. Peraza-Echeverria et al. (2009). Mol Breed. 23:431-443 doi: 10.1007/s11032-008-9247-6	Fusarium wilt tropical race 4 resistance	2,672 bp sense portion of the RGA2 ORF
19	T-nos-RHIRD	Agrobacterium tumefaciens	-	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807
20	P-35S-CaMV	Cauliflower mosaic virus	-			142 bp sense portion of the CaMV35S promoter
21	V-RB-RHIRD	Agrobacterium tumefaciens	-
22	V-LB-RHIRD	Agrobacterium tumefaciens	-
23	T-35S-CaMV	Cauliflower mosaic virus	-	3' untranslated region (3' UTR) of the 35S gene from Cauliflower mosaic virus. Gardner et al. (1981). Nucleic Acids Res, 9:2871-2888
24	CS-nptII-ECOLX	Escherichia coli	-	Neomycin phosphotransferase II gene from the Eschrichia coli Tn5 transposon. Beck et al (1982). Gene, 19, 327-336	Kanamycin/neomycin resistance
25	P-35S-CaMV	Cauliflower mosaic virus	-
26	P-nos-RHIRD	Agrobacterium tumefaciens	-	Promoter region of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983). Nucleic Acids Res 11, 369-385; Fraley et al. (1983). Proc Nat L Acad Sci 80, 4803-4807
27	CS-rga2-MUSAC	Musa acuminata	-	Gene from Musa acuminata conferring resistance to Fusarium wilt tropical race 4. GenBank EU616673/ACF21694. Peraza-Echeverria et al. (2009). Mol Breed. 23:431-443 doi: 10.1007/s11032-008-9247-6	Fusarium wilt tropical race 4 resistance
28	T-nos-RHIRD	Agrobacterium tumefaciens	-	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807
29	V-RB-RHIRD	Agrobacterium tumefaciens	-

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

FSANZ No 26849

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	FSANZ	No	26849

Transformation Vectors

Name:	vector_pSAN3
Comment:
Map:

Genetic Elements:

Reference: FSANZ

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait
1	V-LB-RHIRD	Agrobacterium tumefaciens	26	1-26
2	T-35S-CaMV	Cauliflower mosaic virus	204	93-296	3' untranslated region (3' UTR) of the 35S gene from Cauliflower mosaic virus. Gardner et al. (1981). Nucleic Acids Res, 9:2871-2888
3	CS-nptII-ECOLX	Escherichia coli	798	334-1131	Neomycin phosphotransferase II gene from the Eschrichia coli Tn5 transposon. Beck et al (1982). Gene, 19, 327-336	Kanamycin/neomycin resistance
4	P-35S-CaMV	Cauliflower mosaic virus	808	1162-1969
5	P-nos-RHIRD	Agrobacterium tumefaciens	184	2202-2385	Promoter region of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983). Nucleic Acids Res 11, 369-385; Fraley et al. (1983). Proc Nat L Acad Sci 80, 4803-4807
6	CS-rga2-MUSAC	Musa acuminata	3699	2392-6090	Gene from Musa acuminata conferring resistance to Fusarium wilt tropical race 4. GenBank EU616673/ACF21694. Peraza-Echeverria et al. (2009). Mol Breed. 23:431-443 doi: 10.1007/s11032-008-9247-6	Fusarium wilt tropical race 4 resistance
7	T-nos-RHIRD	Agrobacterium tumefaciens	302	6110-6411	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807
8	V-RB-RHIRD	Agrobacterium tumefaciens	26	6677-6702

Related DNA Sequences

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	FSANZ	No	6702

Detection Methods

22 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-ELE-npt 1-5'/npt 1-3'	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative real-time PCR (TaqMan) method for detection of the nptII gene (Scholtens et al., 2013).	in-house validation	none	1
QL-ELE-P-nos-F/P-nos-R	element-specific	P-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter of Agrobacterium tumefaciens (Debode et al., 2013)	in-house validation	none	1
QL-ELE-T-35S-F/T-35S-R	element-specific	T-Cauliflower mosaic virus	Qualitative real-time PCR (TaqMan) method for detection of the 35S terminator from Cauliflower mosaic virus (Debode et al., 2013)	in-house validation	none	1
QL-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	1
QL-ELE-00-002	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative conventional PCR method for detection of neomycin phosphotransferase II gene (ISO 21569)	ring trial validation	ISO standard	1
QL-ELE-00-003	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative conventional PCR method for detection of the neomycin phosphotransferase II gene (nptII) (BVL L 00.00-31, 2001)	ring trial validation	national standard	1
QL-ELE-00-004	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Lipp et al., 2001)	ring trial validation	ISO standard	1
QL-ELE-00-005	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (ISO/FDIS 21569)	ring trial validation	ISO standard	1
QL-ELE-00-006	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	1
QL-ELE-00-007	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998)	ring trial validation	national standard	1
QL-ELE-00-008	element-specific	P-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter (ISO/TS 21569-4)	ring trial validation	ISO standard	1
QL-ELE-00-009	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001)	ring trial validation	ISO standard	1
QL-ELE-00-011	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1).	ring trial validation	ISO standard	1
QL-ELE-00-012	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1)	ring trial validation	ISO standard	1
QL-ELE-00-013	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1)	ring trial validation	ISO standard	1
QL-ELE-00-017	element-specific	P-Cauliflower mosaic virus	Qualitative real-time PCR (SYBRGreen) method for detection of P-35S CaMV (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-018	element-specific	T-nos-RHIRD	Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-023	element-specific	T-Cauliflower mosaic virus	Qualitative real-time PCR (SYBRGreen) method for detection of T-35S pCAMBIA sequences (EU-RL GMFF GMOMETHODS database as of January 20, 2017).	ring trial validation	EU reference method	1
QT-ELE-NOS ter 2-5' /NOS ter 2-3'	element-specific	T-nos-RHIRD	Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009)	ring trial validation	none	1
QT-ELE-P35S 1-5'/P35S 1-3'	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of the Cauliflower mosaic virus 35S promoter (Kodama et al., 2009)	ring trial validation	national standard	1
QT-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (Feinberg et al., 2005)	ring trial validation	unknown	1
QT-ELE-00-004	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1)	ring trial validation	ISO standard	1

European GMO Initiative for a Unified Database System

GMO detailed information

GMO:

Alias:

UID:

Tradename:

Companies/Institutions:

Species:

Traits:

EU authorisation:

General Information

Links regarding to this GMO:

Inserts / Modification

Transformation Vectors

Related DNA Sequences

Detection Methods

Reference Materials

Authorisation

Literature reference