GMO molecular characterization details

GMO detailed information

GMO:
COR23134
Alias:
UID:
COR-23134-4
Tradename:
Companies/Institutions:
Corteva Agriscience, Pioneer Hi-Bred International
Species:
Glycine max
(soybean)
Traits:
EU authorisation:

no Reference materials could be found

General Information

Description:

The soybean event COR23134 was developed by Agrobacterium-mediated transformation with plasmid PHP90315. Aim of the modification is a triple resistance to certain Lepidoptera and a herbicide selection marker.

The inserted T-DNA carries 4 expression cassettes:

a cry1B.34.1 cassette (CS-cry1B_1Ca1-SYNTH) containing a chimeric gene comprised of modified sequences of B. thuringensis-derived cry genes (cry1B and cry1Ca1), to confer resistance against certain Lepidoptera (e.g. Fall armyworm),
a cry1B.61.1 cassette (CS-cry1B_61_1-BACTU) encoding a modified Cry1B class insecticidal protein which confers resistance against certain Lepidoptera,
an ipd083Cb cassette (CS-ipd083Cb-ADITR), encoding a plant-derived insecticidal protein to confer resistance against Lepidoptera,
a hra_1 cassette (CS-ahas-SOYBN), encoding for a modified acetolactate synthase (ALS) enzyme to confer resistance against ALS inhibiting herbicides and was used as selection marker.

Southern-by-sequencing analysis indicated the integration of one copy of the T-DNA at a single genomic locus and suggested that vector backbone sequences were not integrated.

Sources:

Min Environment Japan: Application for approval (automatic translation into English)
Canada Food Inspection Agency: Notice of submission
Patent WO2025165829A1

Links regarding to this GMO:

Min Environment Japan https://www.env.go.jp/content/000227896.pdf,

Canada Food Inspection Agency https://inspection.canada.ca/en/plant-varieties/plants-novel-traits/notices-submission/cor23134,

Patent WO2025165829A1 https://worldwide.espacenet.com/patent/search/family/096591199/publication/WO2025165829A1?q=WO%202025%2F165829%20A1

Transformation / Modification technique:

Agrobacterium-induced

Inserts / Modification

Name:	insert_COR23134
Comment:	T-DNA region of plasmid PHP90315 indicating the 4 gene cassettes (cry1B.34.1, cry1B.61.1, ipd083Cb, gm-hra_1) intended for incorporation into the soy genome.
Map:

Genetic Elements:

Reference: patent documents

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait	Comment
1	V-RB-RHIRD	Agrobacterium tumefaciens	25	1-25
2	T-glb1-MAIZE	Zea mays	994	90-1083	3' untranslated region (3' UTR) of the globulin 1 gene. Belanger and Kriz (1989) Plant Phys. 91:636-643.		additional terminator sequence
3	T-ubi1-ORYSA	Oryza sativa	950	1395-2344	3'UTR of the ubiquitin 1 gene from Oryza sativa. Phytozome ID Os06g46770, Wang et al (2000) Plant Sci 156(2),201-211. doi:10.1016/s0168-9452(00)00255-7.
4	CS-cry1B_1Ca1-SYNTH	Synthetic	1998	2351-4348	Chimeric gene comprised of modified sequences originating from two Bacillus thuringiensis genes: a cry1B-class gene and cry1Ca1.	Lepidoptera resistance
5	I-1_ubi1-MAIZE	Zea mays	1095	4378-5472			including 5' untranslated region (82 bp, Pos. 5391-5472)
6	P-H2B-MAIZE	Zea mays	1452	5497-6948	Promoter of the histone gene H2B. Joanin et al. (1992). Plant Mol Biol, 20:581-588.		GenBank accession NM_001196058.2
7	T-ubi-SORBI	Sorghum bicolor	584	7195-7778	Terminator of the ubiquitin gene. Phytozome gene ID Sobic.004G049900.l.		additional terminator to prevent transcriptional interference
8	T-act1-SORBI	Sorghum bicolor	1043	7785-8827	3' untranslated region (3' UTR) of actin1 gene from Sorghum bicolor. XM_002441128.		additional terminator to prevent transcriptional interference
9	T-os-T28-SYNTH	Synthetic	881	8938-9818	Synthetic bidirectional terminator region from a NAC domain-containing gene (Phytozome gene ID LOC_Os03g60080.1) and the methylenetetrahydrofolate reductase gene (Phytozome gene ID LOC_Os03g60090.1) from Oryza sativa.
10	CS-cry1B_61_1-BACTU	Bacillus thuringiensis	1971	9826-11796	Modified cry1B-class gene derived from Bacillus thuringiensis.	Lepidoptera resistance
11	P-cab3-SOYBN	Glycine max	1980	11813-13792	Promoter and leader sequence of chlorophyll a/b binding 3 gene (cab3) from Glycine max. Phytozome gene ID Glyma05g25810, US Patent 20200407742.		including 5' untranslated region (125 bp, Pos. 11813-11937)
12	T-pepc1-SORBI	Sorghum bicolor	997	14067-15063	Terminator region from the phosphoenolpyruvate carboxylase 1 (pepc1) gene from Sorghum bicolor. Patent WO2018102131A1		additional terminator to prevent transcriptional interference
13	T-ubi3-ARATH	Arabidopsis thaliana	1084	15087-16170	3' untranslated region (3' UTR) of the ubiquitin 3 gene. Callis et al. (1995). Genetics, 139:921-939.		additional terminator to prevent transcriptional interference
14	T-os-T17-SYNTH	Synthetic	776	16334-17109	Synthetic bidirectional terminator region from a 5-enolpyruvyl shikimate-3-phosphate synthase gene (Phytozome gene ID LOC_Os06g04280.1) and the ribosomal protein S10 gene (Phytozome gene ID LOC_Os06g04290.1) from Oryza sativa.
15	CS-ipd083Cb-ADITR	Adiantum trapeziforme	2562	17117-19678	A fern-derived gene encoding an insecticidal protein which confers resistance against certain Lepidoptera. GenBank KY558370.1; Patent US10227608.	Lepidoptera resistance
16	I-ubi2-PHAVU	Phaseolus vulgaris	287	19698-19984	Intron of the ubiquitin 2 gene from Phaseolus vulgaris. Phytozome gene ID Phvul.003G123900.1.
17	P-ubi2-PHAVU	Phaseolus vulgaris	2683	19985-22667	Promoter of the ubiquitin 2 gene from Phaseolus vulgaris. Phytozome gene ID Phvul.003G123900.1.		including 5' untranslated region (108 bp, Pos. 19985-20092)
18	P-sams-SOYBN	Glycine max	698	22845-23542	Promoter of the S-adenosyl-L-methionine synthetase gene. Falco and Li (2007, 2010). Patent: US 7,217,858 B2; Patent US7834242 B2.		including a part of the 5' untranslated region (59 bp, Pos. 23484-23542)
19	I-sams-SOYBN	Glycine max	605	23543-24147	Intron of an S-adenosyl-L-methionine synthetase gene from Glycine max. US Patent 7834242.		including a part of the 5' untranslated region (14 bp, Pos. 23134-24147)
20	CS-ahas-SOYBN	Glycine max	1956	24215-26170	Herbicide-resistant acetohydroxy acid synthase gene (mutated). Sebastian et al. (1989). Crop Sci, 29:1403-1408; Sebastian (1992). US5084082A; Tan et al. (2005). Pest manag Sci, 61:246-257.	Sulfonylurea tolerance
21	T-ahas-SOYBN	Glycine max	652	26171-26822	3' untranslated region (3' UTR) of the native acetohydroxy acid synthase gene from Glycine max. Ghio et al. (2013) Theor Appl Genet 126:2957-2968.
22	T-z19-MAIZE	Zea mays	690	26980-27669	Terminator of the 19-kDA zein gene from Zea mays. GenBank KX247647.		additional terminator sequence
23	V-LB-RHIRD	Agrobacterium tumefaciens	25	27777-27801

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

patent documents No

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	patent documents	No

Detection Methods

12 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-TAX-GM-001	taxon-specific	Glycine max	Qualitative real-time PCR (SYBRGreen) method for detection of soybean lectin gene (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-TAX-GM-002	taxon-specific	Glycine max	Qualitative conventional PCR method for detection of soybean lectin gene (ISO/FDIS 21569).	ring trial validation	ISO standard	1
QL-TAX-GM-008	taxon-specific	Glycine max	Qualitative conventional PCR method for detection of soybean lectin gene (ISO 21569)	ring trial validation	ISO standard	1
QT-EVE-GM-021	event-specific	COR23134	Quantitative real-time PCR (TaqMan) method for detection of soybean event COR23134 (EU-RL GMFF GMOMETHODS database as of August 5, 2025).	ring trial validation	EU reference method	1
QT-TAX-GM-001	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene	ring trial validation	ISO standard	1
QT-TAX-GM-002	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (ISO 21570). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	ISO standard	1
QT-TAX-GM-003	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of July 2020). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-GM-004	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (Hird et al., 2003).	ring trial validation	unknown	1
QT-TAX-GM-005	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) for detection of soybean lectin gene (EU-RL GMFF GMOMETHODS database as of November 8, 2023).	ring trial validation	EU reference method	1
QT-TAX-GM-007	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene	ring trial validation	ISO standard	1
QT-TAX-GM-009	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014)	ring trial validation	EU reference method	1
QT-TAX-GM-020	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1

Citation	Publication year	Publishing authority	Publication type	Content types
EU-RL GMFF GMOMETHODS database; QT-EVE-GM-021	2025	JRC	EU reference method protocol	detection
Application for authorisation of genetically modified plants and derived food and feed in accordance with Regulation (EC) No 1829/2003. GMFF-2023-21132 AP192, Part VII – Summary of Application	2024	EFSA	application summary	application summary

European GMO Initiative for a Unified Database System

GMO detailed information

GMO:

Alias:

UID:

Tradename:

Companies/Institutions:

Species:

Traits:

EU authorisation:

General Information

Links regarding to this GMO:

Inserts / Modification

Transformation Vectors

Detection Methods

Reference Materials

Authorisation

Literature reference