Description:
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GE-Vinv Potato lines contain a single gene knock-out of the endogenous vacuolar acid invertase gene Vinv achieved through transient expression of a Transcription Activator-Like Effector Nuclease (TALEN).
GE-Vinv Potato lines have improved consumer safety and processing characteristics. Tubers accumulate a reduced amount of reducing sugars during cold storage (reduced cold-induced sweetening), leading to a reduction of acrylamide formation upon high-temperature processing.
The potato genome contains only one Vinv gene, but because cultivated potato is a tetraploid, multiple alleles of Vinv are present in each variety. Three TALEN pairs were designed to target the _Vinv gene family within the first 200 bp of the coding sequence. The TALEN encoding plasmids were individually introduced into potato protoplasts by polyethylene glycol (PEG)-mediated transformation. They were transiently expressed in these protoplasts, resulting in a targeted deletion to achieve the knock-out of the endogenous Vinv gene. Different lines with mutations in one or more alleles of the Vinv gene were created. No selectable marker system was used. PCR analyses confirmed the absence of TALEN-derived DNA or integration of the expression plasmid into the genome of the selected Vinv Potato lines.
Source: Patent WO 2014/096972
The GE-Vinv Potato line St116-1 was developed using the potato variety Ranger Russet. It contains a frameshift-inducing mutation (ranging from 4 to 17 bp deletions) in each of the four Vinv alleles.
Source: Patent WO 2014/096972; Clasen et al. (2016)
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Links regarding to this GMO:
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Clasen et al. (2016) http://onlinelibrary.wiley.com/doi/epdf/10.1111/pbi.12370,
Patent http://patentscope.wipo.int/search/en/detail.jsf?docId=WO2014096972&redirectedID=true
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Transformation / Modification technique:
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Transcription activator-like effector nucleases (TALEN)
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