Description:
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Ms11 was produced by means of Agrobacterium-mediated transformation using the vector pTC0113.
Southern blot results demonstrated the presence of one complete T-DNA insert containing the bar, the barnase and the barstar gene cassettes in Ms11 B. napus.
The hybrid technology comprises three components:
a dominant gene for male sterility - the
barnase gene (event Ms11),
a dominant gene for fertility restoration - the barstar gene (event Rf3) and
the bar gene (found in both Ms11 and Rf3) conferring tolerance to glufosinate-ammonium.
Ms11 is a male sterile line that segregates 1:1 for sterility and fertility and is only used for the production of the Ms11xRf3 B. napus hybrid seed. According to Bayer, it will never be commercialized as a stand-alone product.
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Links regarding to this GMO:
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USDA-APHIS http://www.aphis.usda.gov/brs/aphisdocs/16_23501p.pdf,
BCH https://bch.cbd.int/en/database/record?documentID=112988
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Transformation / Modification technique:
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Agrobacterium-induced
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