QL-CON-00-005 |
construct-specific |
32316, 40416, 4114, 43A47, 676, 678, 680, A2704-12, A2704-21, A5547-127, A5547-35, DAS1507, DAS59122, DAS59132, DBN9004, DP62151, DP910521, Falcon GS 40/90, GU262, HCN10, HCR-1, Liberator, T120-7, T14, T25, T45, Topas 19/2 |
Qualitative conventional PCR method for detection of maize event T25 (ISO 21569) |
ring trial validation |
ISO standard |
1 |
QL-CON-00-011 |
construct-specific |
32316, 40416, 4114, 43A47, 676, 678, 680, A2704-12, A2704-21, A5547-127, A5547-35, Bt10 maize, Bt11, DAS1507, DAS59122, DAS59132, DBN9004, DHA, Falcon GS 40/90, GU262, HCN10, HCR-1, Liberator, MZHG0JG, MZIR098, SYHT0H2, T120-7, T14, T25, T45, Topas 19/2 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the 35S promoter and the phosphinothricin N-acetyltransferase (pat) gene (ISO 21569-3). |
ring trial validation |
ISO standard |
3 |
QL-CON-00-014 |
construct-specific |
1345-4, 16-0-1, 18-2-4, 35 1 N, 55-1, 5B, 63-1, A1-DFR Petunias, B tomato, C5, Cantaloupe A, Cantaloupe B, Da tomato, EH92-527-1, F tomato, F3'5'H GM petunia, FP967, GD743, GFM Cry1A, GK19, GS784, H421, HCN10, HLMT15-15, HLMT15-3, HLMT15-46, Huafan No 1, Huanong No. 1, INI-FMAPDF, KMD1, MLS 9124, MPS965, Ms1, NF872, NXI-1T, NXI-4T, NXI-6T, PG451, RBMT15-101, RBMT21-129, RBMT21-152, RBMT21-350, RL01-15, RL01-17, RM3-3, RM3-4, RM3-6, Rf1, Rf2, SEMT15-02, SEMT15-15, SGK321, SY233, T120-7, TAM66274, Topas 19/2, X17-2 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the nopaline synthase promoter (P-nos)and the neomycin phosphotransferase II gene (nptII) (ISO/TS 21569-4) |
ring trial validation |
ISO standard |
1 |
QL-ELE-npt 1-5'/npt 1-3' |
element-specific |
CS-nptII-ECOLX, V-nptII-ECOLX |
Qualitative real-time PCR (TaqMan) method for detection of the nptII gene (Scholtens et al., 2013). |
in-house validation |
none |
1 |
QL-ELE-Patf-n2/Patr-n2 |
element-specific |
CS-pat-STRVR |
Qualitative real-time PCR (TaqMan) method for detection of the pat gene (Xu et al., 2006) |
in-house validation |
none |
1 |
QL-ELE-P-nos-F/P-nos-R |
element-specific |
P-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter of Agrobacterium tumefaciens (Debode et al., 2013) |
in-house validation |
none |
1 |
QL-ELE-T-OCS-F/T-OCS-R |
element-specific |
T-ocs-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of octopine synthase terminator of Agrobacterium tumefaciens (Debode et al., 2013) |
in-house validation |
none |
1 |
QL-ELE-T-35S-F/T-35S-R |
element-specific |
T-Cauliflower mosaic virus |
Qualitative real-time PCR (TaqMan) method for detection of the 35S terminator from Cauliflower mosaic virus (Debode et al., 2013) |
in-house validation |
none |
1 |
QL-ELE-00-001 |
element-specific |
P-Cauliflower mosaic virus |
Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Official Collection of Methods according to § 64 LFGB, L 00.00-31) |
ring trial validation |
national standard |
1 |
QL-ELE-00-002 |
element-specific |
CS-nptII-ECOLX, V-nptII-ECOLX |
Qualitative conventional PCR method for detection of neomycin phosphotransferase II gene (ISO 21569) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-003 |
element-specific |
CS-nptII-ECOLX, V-nptII-ECOLX |
Qualitative conventional PCR method for detection of the neomycin phosphotransferase II gene (nptII) (BVL L 00.00-31, 2001) |
ring trial validation |
national standard |
1 |
QL-ELE-00-004 |
element-specific |
P-Cauliflower mosaic virus |
Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Lipp et al., 2001) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-005 |
element-specific |
P-Cauliflower mosaic virus |
Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (ISO/FDIS 21569) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-008 |
element-specific |
P-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter (ISO/TS 21569-4) |
ring trial validation |
ISO standard |
3 |
QL-ELE-00-012 |
element-specific |
E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH |
Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S).
Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-017 |
element-specific |
P-Cauliflower mosaic virus |
Qualitative real-time PCR (SYBRGreen) method for detection of P-35S CaMV (Barbau-Piednoir et al., 2014) |
ring trial validation |
unknown |
1 |
QL-ELE-00-021 |
element-specific |
CS-pat-STRVR |
Qualitative real-time PCR (SYBRGreen) method for detection of CS-pat-STRVR (Barbau-Piednoir et al., 2014) |
ring trial validation |
unknown |
1 |
QL-ELE-00-023 |
element-specific |
T-Cauliflower mosaic virus |
Qualitative real-time PCR (SYBRGreen) method for detection of T-35S pCAMBIA sequences (EU-RL GMFF GMOMETHODS database as of January 20, 2017). |
ring trial validation |
EU reference method |
1 |
QL-ELE-00-025 |
element-specific |
CS-pat-STRVR |
Qualitative real-time PCR (TaqMan) method for detection of the synthetic phosphinothricin acetyltransferase (pat) gene (BVL L00.00-154). EU-RL GMFF GMOMETHODS database as of December 23, 2016. |
ring trial validation |
EU reference method |
1 |
QT-CON-00-005 |
construct-specific |
32316, 40416, 4114, 43A47, 676, 678, 680, A2704-12, A2704-21, A5547-127, A5547-35, DAS1507, DAS59122, DAS59132, DBN9004, DP62151, DP910521, Falcon GS 40/90, GU262, HCN10, HCR-1, Liberator, T120-7, T14, T25, T45, Topas 19/2 |
Quantitative real-time PCR (TaqMan) method for detection of maize event T25 (Shindo et al., 2002) |
ring trial validation |
ISO standard |
1 |
QT-ELE-P35S 1-5'/P35S 1-3' |
element-specific |
P-Cauliflower mosaic virus |
Quantitative real-time PCR (TaqMan) method for detection of the Cauliflower mosaic virus 35S promoter (Kodama et al., 2009) |
ring trial validation |
national standard |
1 |
QT-ELE-00-001 |
element-specific |
P-Cauliflower mosaic virus |
Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (Feinberg et al., 2005) |
ring trial validation |
unknown |
1 |
QT-ELE-00-002 |
element-specific |
CS-pat-STRVR |
Quantitative real-time PCR (TaqMan) method for detection of phosphinothricin N-acetyltransferase gene (Weighardt et al., 2004) (EURL GMFF GMOMETHODS database as of February 5, 2020) |
ring trial validation |
unknown |
1 |
QT-ELE-00-004 |
element-specific |
E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH |
Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1) |
ring trial validation |
ISO standard |
3 |
QT-TAX-BV-013 |
taxon-specific |
Beta vulgaris |
Quantitative real-time PCR (TaqMan) method for detection of sugar beet glutamine synthetase (GS2) gene (EU-RL GMFF GMOMETHODS database as of March 05, 2014) |
ring trial validation |
EU reference method |
1 |