Description:
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Canola event 23-18-17 was created by Agrobacterium-mediated transformation from the 212/86 canola variety. The transfer-DNA (T-DNA) contained:
- the gene encoding the enzyme 12:0 ACP thioesterase (bay TE) from the California bay tree (Umbellularia californica). Bay TE is active in the fatty acid biosynthetic pathway of the developing seed resulting in the accumulation of triacylglycerides containing esterified lauric acid (12:0) and, to a lesser extent, myristic acid (14:0).
- sequences encoding the enzyme neomycin phosphotransferase II (NPTII). The expression of NPTII activity was used as a selectable trait.
Southern blot analysis and segregation analysis indicated that the original transformation event had insertions at five independent genomic loci. However, line 23-18-17 which is an offspring of the original transformant may have lost some of the original copies due to segregation.
Line 23-18-17 is a sister event of Canola event 23-198.
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Links regarding to this GMO:
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USDA-APHIS http://www.aphis.usda.gov/brs/aphisdocs/94_09001p.pdf,
BCH http://bch.cbd.int/database/record.shtml?documentid=14780,
ISAAA http://www.isaaa.org/gmapprovaldatabase/event/default.asp?EventID=8
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Transformation / Modification technique:
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Agrobacterium-induced
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