Description:
|
The Thlapsi arvense event 'FAE1 Pennycress' was developed by targeted indel mutations in the FAE1 (Fatty Acid Elongation) gene of Thlaspi arvense, thereby abolishing erucic acid production and creating an edible seed oil.
The Illinois State University has introduced CRISPR/Cas9 mediated FAE loss-of-function mutations ~215 bp down stream of the TaFAE1 translational start site.
This was accomplished using the TaFAE1-CRISPR Cas9_ Hyg vector which was introduced using Agrobacterium-mediated vacuum infiltration technique. CRISPR/Cas9 succes was determined using T7 endonucleases.
In the described knock-out lines the CRISPR/Cas9 DNA construct was segregated away. It was undetectable by PCR.
This process created 3 homozygous lines with different mutations:
- fae1-3 (4 bp deletion)
- fae1-4 (single A insertion)
- fae1-5 (single T insertion)
|
Links regarding to this GMO:
|
Publication describing the creation of FAE1 Pennycress http://onlinelibrary.wiley.com/doi/epdf/10.1111/pbi.13014,
USDA non-regulation status inquiry http://www.aphis.usda.gov/biotechnology/downloads/reg_loi/18-036-01_a1_air_inquiry_cbidel.pdf,
USDA non-regulation status confirmation http://www.aphis.usda.gov/biotechnology/downloads/reg_loi/18-036-01_air_response_signed.pdf
|
Transformation / Modification technique:
|
CRISPR/Cas
|