Description:
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The GE-GGP2 lettuce events were generated by editing the upstream open reading frame (uORF) of
GDP-L-galactose phosphorylase (GGP2), a key enzyme in vitamin C biosynthesis.
A CRISPR/Cas9 construct, with a single-guide RNA that targeted the region harboring a uORF initiation codon, was delivered into cotyledon explants of lettuce with Agrobacterium tumefaciens.
Three regenerated mutant lines were analysed; two showed small deletions (up to 14 bp) present
proximal to the initiation codon (ACG), but a large deletion (92 bp) in the third line led to the loss of both the initiation codon and a portion of the uORF coding region.
All three mutant lines showed not only increased oxidation stress tolerance, but also significantly increased foliar ascorbate content.
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Links regarding to this GMO:
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Scientific journal https://www.nature.com/articles/nbt.4202
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Transformation / Modification technique:
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CRISPR/Cas
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