Description:
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GE-PPO Potato contains a transgene-free single gene, multi-allele knockout mutation of a polyphenol oxidase (ppo) gene achieved using Transcription Activator-Like Effector Nuclease (TALEN). The TALEN reagent was introduced by polyethylene glycol (PEG)-mediated transformation of potato protoplasts and was transiently expressed in the protoplasts, resulting in targeted deletions to achieve the gene knockout of an endogenous ppo gene. No selectable marker system was used.
GE-PPO Potato has improved processing characteristics.
PCR analyses confirmed the absence of TALEN-derived DNA or integration of the expression plasmid into the genome of GE-PPO Potato.
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Links regarding to this GMO:
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USDA-APHIS http://www.aphis.usda.gov/biotechnology/downloads/reg_loi/16-090-01_air_inquiry_cbidel.pdf
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Transformation / Modification technique:
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Transcription activator-like effector nucleases (TALEN)
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