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Detection Image Detection method details

Name:

QT-TAX-ZM-002

Description:

Quantitative real-time PCR (TaqMan) method for detection of the maize high-mobility-group A (hmgA) gene (ISO 21570).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

Comment:

Target is the endogenous high-mobility-group A (hmgA) gene from Zea mays. This assay is recommended for quantitative GMO analysis.

Validation:

ring trial validation

Standardisation:

ISO standard

Related Methods:

Type:

taxon-specific

Target Species name:

Zea mays
  • Oligonucleotides:

  • Forward Primer

  • Name:

    ZM1-F
  • Sequence:

    TTGGACTAGAAATCTCGTGCTGA
  • Size:

    23
  • Reverse Primer

  • Name:

    ZM1-R
  • Sequence:

    GCTACATAGGGAGCCTTGTCCT
  • Size:

    22
  • Probe

  • Name:

    Probe ZM1
  • Sequence:

    CAATCCACACAAACGCACGCGTA
  • Size:

    23
  • Amplicon:

  • Sequence:

    ttggactagaaatctcgtgctgannnnnnnnnntacgcgtgcgtttgtgtggattgnaggacaaggctccctatgtagc
  • Size:

    79

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-TAX-ZM-002Link to the document url. EU reference method protocol
Jacchia S., Kagkli D.F., Lievens A., Angers-Loustau A., Savini C., Emons H., Mazzara M. (2018) Identification of single target taxon-specific reference assays for the most commonly genetically transformed crops using digital droplet PCR. Food Control 93,191-200Link to the document url. peer-reviewed article 31-10-2018
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Quantitative nucleic acid based methods" ISO 21570:1-103 (2005)Link to the document url.