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Detection Image Detection method details

Name:

QT-TAX-GH-018

Description:

Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of January 17, 2014).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

Comment:

Target is the endogenous alcohol dehydrogenase C (adhC) gene from Gossypium hirsutum.

This assay is recommended for GMO quantitative analysis.

Validation:

ring trial validation

Standardisation:

EU reference method

Related Methods:

Type:

taxon-specific

Target Species name:

Gossypium hirsutum
  • Oligonucleotides:

  • Forward Primer

  • Name:

    KVM157
  • Sequence:

    CACATGACTTAGCCCATCTTTGC
  • Size:

    23
  • Reverse Primer

  • Name:

    KVM158
  • Sequence:

    CCCACCCTTTTTTGGTTTAGC
  • Size:

    21
  • Probe

  • Name:

    TM012
  • Sequence:

    TGCAGGTTTTGGTGCCACTGTGAATG
  • Size:

    26
  • Amplicon:

  • Sequence:

    cacatgacttagcccatctttgcntgcaggttttggtgccactgtgaatgnngctaaaccaaaaaagggtggg
  • Size:

    73

Documents

Citation Type Local copy
Jacchia S., Kagkli D.F., Lievens A., Angers-Loustau A., Savini C., Emons H., Mazzara M. (2018) Identification of single target taxon-specific reference assays for the most commonly genetically transformed crops using digital droplet PCR. Food Control 93,191-200Link to the document url. peer-reviewed article 31-10-2018
EU-RL GMFF GMOMETHODS database; QT-TAX-GH-018Link to the document url. EU reference method protocol