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Detection Image Detection method details

Name:

QT-TAX-CP-001

Description:

Quantitative real-time (TaqMan) PCR method for detection of papaya chymopapain (chy) gene (EU-RL GMFF GMOMETHODS database as of September 13, 2016)

Comment:

Target is the endogenous chymopapain (chy) gene from papaya (Carica papaya)

Validation:

ring trial validation

Standardisation:

EU reference method

Related Methods:

Type:

taxon-specific

Target Species name:

Carica papaya
  • Oligonucleotides:

  • Forward Primer

  • Name:

    Q-Chy-1F
  • Sequence:

    ccatgcggatcctccca
  • Size:

    17
  • Reverse Primer

  • Name:

    Q-Chy-2R
  • Sequence:

    catcgtagccattgtaacactagctaa
  • Size:

    27
  • Probe

  • Name:

    Q-Chy-P
  • Sequence:

    ttcccttcatccattcccactcttgaga
  • Size:

    28
  • Amplicon:

  • Sequence:

    ccatgcggatcctcccatttcccttcatccattcccactcttgagagttagctagtgttacaatggctacgatg
  • Size:

    74

Documents

Citation Type Local copy
Guo, J. C., Yang, L. T., Liu, X., Guan, X. Y., Jiang, L. X., & Zhang, D. B. (2009). Characterization of the exogenous insert and development of event-specific PCR detection methods for genetically modified Huanong no. 1 papaya. Journal of Agricultural and Food Chemistry, 57(16), 7205-7212.Link to the document url. peer-reviewed article 10-10-2016
Wei, J. et al. (2016). Collaborative trial for the validation of event-specific PCR detection methods of genetically modified papaya Huanong No. 1. Food Chemistry, 194, 20-25.Link to the document url. peer-reviewed article 14-10-2016