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Detection Image Detection method details

Name:

QT-EVE-ZM-025

Description:

Quantitative PCR method for detection of maize event MON 87403 (EURL GMFF, GMOMETHODS database, as of May 09, 2018)

Comment:

Target is the 3' integration border region (IBR) between the insert of maize event MON87403 and the maize host genome.

Validation:

ring trial validation

Standardisation:

EU reference method

Related Methods:

QT-TAX-ZM-002

Type:

event-specific

Target GMO name:

MON87403
  • Oligonucleotides:

  • Forward Primer

  • Name:

    87403 QF
  • Sequence:

    CTTTCTTTTTCTCCATATTGACCATCATAC
  • Size:

    30
  • Reverse Primer

  • Name:

    87403 QR
  • Sequence:

    TACTCCGGAATGAGTGCTCTGTATC
  • Size:

    25
  • Probe

  • Name:

    87403 QP
  • Sequence:

    TCATTGCGATCCACATTTCCCTACATGG
  • Size:

    28
  • Amplicon:

  • Sequence:

    ctttcttttt ctccatattg accatcatac tcattgcgat ccacatttcc ctacatggnn nnngatacag agcactcatt ccggagta
  • Size:

    88

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-025Link to the document url. EU reference method protocol 09-05-2018