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Detection Image Detection method details

Name:

QT-CON-00-007

Description:

Quantitative real-time PCR (TaqMan) method for detection of maize event Bt176 (Shindo et al., 2002)

Comment:

Target is the junction region between a synthetic cryIA(b) gene and the phospho-enol-pyruvate carboxylase (PEPC) intron N.9

Validation:

ring trial validation

Standardisation:

ISO standard

Related Methods:

QT-TAX-ZM-006

Type:

construct-specific

Target GMO names:

Bt176
  • Oligonucleotides:

  • Forward Primer

  • Name:

    E176 2-5'
  • Sequence:

    TGTTCACCAGCAGCAACCAG
  • Size:

    20
  • Reverse Primer

  • Name:

    E176 2-3'
  • Sequence:

    ACTCCACTTTGTGCAGAACAGATCT
  • Size:

    25
  • Probe

  • Name:

    E176-Taq
  • Sequence:

    CCGACGTGACCGACTACCACATCGA
  • Size:

    25
  • Amplicon:

  • Sequence:

    tgttcaccagcagcaaccagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagatctgttctgcacaaagtggagt
  • Size:

    100

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-CON-00-007Link to the document url. EU reference method protocol
Shindo Y., Kuribara H., Matsuoka T., Futo S., Sawada C., Shono J., Akiyama H., Goda Y., Toyoda M., Hino A.; Validation of real-time PCR analyses for line-specific quantitation of genetically modified maize and soybean using new reference molecules J AOALink to the document url. peer-reviewed article
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Quantitative nucleic acid based methods ISO 21570:1-103 (2005)Link to the document url. other document