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Detection Image Detection method details

Name:

QL-ELE-00-010

Description:

Qualitative conventional PCR method for detection of Figwort mosaic virus 34S promoter (Pan et al., 2007)

Comment:

Target is the Figwort mosaic virus 34S promoter (P-FMV)

Validation:

ring trial validation

Standardisation:

ISO standard

Related Methods:

Type:

element-specific

Target DNA element:

P-FMV
  • Oligonucleotides:

  • Forward Primer

  • Name:

    FMV-1
  • Sequence:

    AAGCCTCAACAAGGTCAG
  • Size:

    18
  • Reverse Primer

  • Name:

    FMV-2
  • Sequence:

    CTGCTCGATGTTGACAAG
  • Size:

    18
  • Amplicon:

  • Sequence:

    aagcctcaacaaggtcagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncttgtcaacatcgagcag
  • Size:

    196

Documents

Citation Type Local copy
Pan L., Zhang S., Yang L., Broll H., Tian F., Zhang D.; Interlaboratory trial validation of an event-specific qualitative polymerase chain reaction-based detection method for genetically modified RT73 rapeseed J AOAC Int 90:1639-1646 (2007)Link to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QL-ELE-00-010Link to the document url. EU reference method protocol