Help icon

Detection Image Detection method details

Name:

 QL-CON-GH-tFreeze-F/GH-tFreeze-R

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the junction between the growth hormone gene and the antifreeze terminator present in AquAdvantage Salmon (Debode et al., 2018)

Comment:

 Target is the junction region between the growth hormone gene (CS-GHc2) sequence from Oncorhynchus tshawytscha and the antifreeze terminator (T-AFP) from Zoarces americanus

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 construct-specific

Target GMO names:
AquAdvantage Salmon

  • Oligonucleotides:

  • Forward Primer

  • Name:

    GH-tFreeze-F

  • Sequence:

    ctccacaggttttgacatgttca

  • Size:

    23

  • Reverse Primer

  • Name:

    GH-tFreeze-R

  • Sequence:

    gccagcaagagcccatctc

  • Size:

    19

  • Probe

  • Name:

    GH-tFreeze-P

  • Sequence:

    ttcctaatctgtatctgggaaaccgaaccct

  • Size:

    31

  • Amplicon:

  • Sequence:

    ctccacaggttttgacatgttcannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngagatgggctcttgctggc

  • Size:

    75

Documents

Citation Type Local copy
Protocol QL-CON-GH-tFreeze-F/GH-tFreeze-R, Debode et al. 2017 PCR Protocol pdf
Debode F., Janssen E., Marien A. et al. (2018) Detection of transgenic atlantic and Coho Salmon by real-time PCR. Food Analytical Methods,11(9),2396–2406Link to the document url. peer-reviewed article 31-10-2018