Detection method details
Name:
QL-CON-GH-tFreeze-F/GH-tFreeze-RDescription:
Qualitative real-time PCR (TaqMan) method for detection of the junction between the growth hormone gene and the antifreeze terminator present in AquAdvantage Salmon (Debode et al., 2018)Comment:
Target is the junction region between the growth hormone gene (CS-GHc2) sequence from Oncorhynchus tshawytscha and the antifreeze terminator (T-AFP) from Zoarces americanusValidation:
in-house validationStandardisation:
noneRelated Methods:
Type:
construct-specificTarget GMO names:
AquAdvantage Salmon
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Oligonucleotides:
Forward Primer
Name:
GH-tFreeze-FSequence:
ctccacaggttttgacatgttcaSize:
23
Reverse Primer
Name:
GH-tFreeze-RSequence:
gccagcaagagcccatctcSize:
19
Probe
Name:
GH-tFreeze-PSequence:
ttcctaatctgtatctgggaaaccgaaccctSize:
31
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Amplicon:
Sequence:
ctccacaggttttgacatgttcannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngagatgggctcttgctggcSize:
75
Documents
Citation | Type | Local copy |
---|---|---|
Protocol QL-CON-GH-tFreeze-F/GH-tFreeze-R, Debode et al. 2017 | PCR Protocol | ![]() |
Debode F., Janssen E., Marien A. et al. (2018) Detection of transgenic atlantic and Coho Salmon by real-time PCR. Food Analytical Methods,11(9),2396–2406![]() |
peer-reviewed article | 31-10-2018 |