Help icon

Detection Image Detection method details

Name:

QL-CON-00-001

Description:

Qualitative conventional PCR method for detection of soybean event GTS-40-3-2 (ISO 21569)

Comment:

Target is the junction region between the cauliflower mosaic virus 35S promoter (CaMV P-35S) and the chloroplast transit peptide (CTP) sequence from petunia (Petunia hybrida) epsps gene

Validation:

ring trial validation

Standardisation:

ISO standard

Related Methods:

QL-TAX-GM-002

Type:

construct-specific

Target GMO names:

GTS 40-3-2
  • Oligonucleotides:

  • Forward Primer

  • Name:

    p35s-f2
  • Sequence:

    TGATGTGATATCTCCACTGACG
  • Size:

    22
  • Reverse Primer

  • Name:

    petu-r1
  • Sequence:

    TGTATCCCTTGAGCCATGTTGT
  • Size:

    22
  • Amplicon:

  • Sequence:

    tgatgtgatatctccactgacgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnacaacatggctcaagggataca
  • Size:

    172

Documents

Citation Type Local copy
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Qualitative nucleic acid based methods ISO 21569:1-69 (2005)Link to the document url. other document
EU-RL GMFF GMOMETHODS database; QL-CON-00-001Link to the document url. EU reference method protocol