Detection Image Detection methods search result

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QL-BAC-16S-FW/16S-Rev taxon-specific
bacteria
Qualitative conventional PCR for the amplification of the 16S RNA gene from eubacteria and archaebacteria.

This method is meant to enable species identification or assignment of bacterial nucleotide sequence to a taxonomical group of eubacteria and archaebacteria since the target sequence includes variable and highly variable sequences that might serve as signature sequences. Therefore the herein described PCR method should be performed and followed by sequence analysis of the amplified PCR product. (Official Collection of Methods according to § 28b GenTG, G 21.40-1).

ring trial validation national standard
QL-BAC-27f/926R taxon-specific
bacteria
Qualitative conventional PCR for the amplification of the 16S RNA gene from bacteria.

This method is meant to enable species identification or assignment of bacterial nucleotide sequence to a taxonomical group of bacteria since the target sequence includes variable and highly variable sequences that might serve as signature sequences. Therefore the herein described PCR method should be performed and followed by sequence analysis of the amplified PCR product (Official Collection of Methods according to § 28b GenTG, G 21.40-1).

ring trial validation national standard
QL-CON-cptiF2/tNOS-uni-R construct-specific
Kefeng6
Qualitative real-time PCR (TaqMan) method for detection of the junction between the cpti gene and nos terminator (Reiting et al., 2010) in-house validation none
QL-CON-CP1/pFMV2 construct-specific
GT200, GT73, GTSB77, H7-1, J101, J163, MON1445, MON1698, MON87705, MON88302, MON88913, MON89788, RBMT22-082, RBMT22-186, RBMT22-238, RBMT22-262, ZSR500, ZSR502, ZSR503
Qualitative conventional PCR method for detection of the junction between the FMV promoter and the cp4-epsps gene (BVL G30.40-11) ring trial validation national standard
QL-CON-gat/T-pinII-F/gat/T-pinII-R construct-specific
61061, 73496, 98140, DP356043
Qualitative real-time PCR (TaqMan) method for detection of the junction between the gat gene and pinII terminator (Prins et al., 2016) in-house validation national standard
QL-CON-GH-tFreeze-F/GH-tFreeze-R construct-specific
AquAdvantage Salmon
Qualitative real-time PCR (TaqMan) method for detection of the junction between the growth hormone gene and the antifreeze terminator present in AquAdvantage Salmon (Debode et al., 2018) in-house validation none
QL-CON-MDB498 /DPA143 construct-specific
B16 (DLL25), Bt176, CBH351, DBT418, LLCotton25, LLRICE06, LLRICE601, LLRICE604, LLRICE62, MON88701, Ms3, Ms6, T1c-19, T303-3, T304-40, TC6275, W62, W98
Qualitative real-time PCR (TaqMan) method for detection of the junction between the 35S promoter and the bar gene (Bayer CropScience, 2006) in-house validation none
QL-CON-PGS-bar-A2/PGS-bar-B2 construct-specific
Ms1, Ms11, Ms8, RM3-3, RM3-4, RM3-6, Rf1, Rf2, Rf3
Qualitative conventional PCR method for detection of the junction between P-SSU promoter and the bar gene (BVL G30.40-13) ring trial validation national standard
QL-CON-p-nos-F2/NPTII-R construct-specific
1345-4, 16-0-1, 18-2-4, 35 1 N, 55-1, 63-1, B tomato, C5, Da tomato, EH92-527-1, F tomato, F3'5'H GM petunia, FP967, GD743, GFM Cry1A, GS784, HLMT15-15, HLMT15-3, HLMT15-46, Huanong No. 1, INI-FMAPDF, KMD1, MPS965, Melon A, Melon B, Ms1, NF872, RBMT15-101, RBMT21-129, RBMT21-152, RBMT21-350, RL01-15, RL01-17, RM3-3, RM3-4, RM3-6, Rf1, Rf2, SEMT15-02, SEMT15-15, T120-7, Topas 19/2, X17-2, dfr GM petunia
Qualitative real-time PCR (TaqMan) method for detection of the junction between the nopaline synthase promoter (P-nos)and the neomycin phosphotransferase II gene (nptII) (ISO/TS 21569-4) ring trial validation ISO standard
QL-CON-00-001 construct-specific
GTS 40-3-2
Qualitative conventional PCR method for detection of soybean event GTS-40-3-2 (ISO 21569) ring trial validation ISO standard
QL-CON-00-002 construct-specific
Nema282F
Qualitative conventional PCR method for detection of tomato event Nema282F (ISO/FDIS 21569) ring trial validation ISO standard
QL-CON-00-003 construct-specific
Bt10 maize, Bt11
Qualitative conventional PCR method for detection of maize event Bt11 (ISO 21569) ring trial validation ISO standard
QL-CON-00-004 construct-specific
Bt176
Qualitative conventional PCR method for detection of maize event Bt176 (ISO 21569) ring trial validation ISO standard
QL-CON-00-005 construct-specific
32316, 40416, 4114, 678, 680, A2704-12, A2704-21, A5547-127, A5547-35, DAS1507, DAS59122, DAS59132, Falcon GS 40/90, GU262, HCN10, HCR-1, Liberator, T120-7, T14, T25, T45, Topas 19/2
Qualitative conventional PCR method for detection of maize event T25 (ISO 21569) ring trial validation ISO standard
QL-CON-00-006 construct-specific
GTS 40-3-2
Qualitative conventional PCR method for detection of soybean event GTS-40-3-2 (ISO 21569) ring trial validation ISO standard
QL-CON-00-007 construct-specific
Huahui No.1
Qualitative real-time PCR (TaqMan) method for detection of rice event Bt63 (ISO 21569-Amd) ring trial validation ISO standard
QL-CON-00-008 construct-specific
ASR368, GT200, GT73, GTSB77, H7-1, J101, J163, MON1445, MON1698, MON71200, MON71700, MON71800, MON80100, MON802, MON809, MON832, MON87411, MON87427, MON87705, MON88017, MON88302, MON88913, MON89788, NK603, RBMT22-082, RBMT22-186, RBMT22-238, RBMT22-262, ZSR500, ZSR502, ZSR503
Qualitative real-time PCR (TaqMan) method for detection of the junction between the chloroplast transit peptide 2 and the CP4 epsps gene (ISO 21569 AMD1). ring trial validation ISO standard
QL-CON-00-010 construct-specific
FP967
Qualitative real-time PCR (TaqMan) method for detection of a construct present in flax event FP967 (ISO 21569-2) ring trial validation ISO standard
QL-CON-00-011 construct-specific
32316, 40416, 4114, 676, 678, 680, A2704-12, A2704-21, A5547-127, A5547-35, Bt10 maize, Bt11, DAS1507, DAS59122, DAS59132, Falcon GS 40/90, GU262, HCN10, Liberator, MZHG0JG, MZIR098, SYHT0H2, T120-7, T14, T25, T45, Topas 19/2
Qualitative real-time PCR (TaqMan) method for detection of the junction between the 35S promoter and the phosphinothricin N-acetyltransferase (pat) gene (G 30.40-1). ring trial validation national standard
QL-CON-00-012 construct-specific
55-1, 63-1
Qualitative real-time PCR (TaqMan) method for detection of papaya event 55-1 and 63-1 (ISO 21569) ring trial validation ISO standard
QL-CON-00-013 construct-specific
3006-210-23, 4114, DAS1507, DAS59122, DAS59132, KMD1, Kefeng6
Qualitative real-time PCR (TaqMan) method for detection of the junction between the maize ubiquitin promoter and the modified cry1Ab/1Ac genes (Grohmann et al.,2015) ring trial validation EU reference method
QL-CON-307-F/652-R construct-specific
KMD1, Kefeng6
Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the hpt gene (Reiting et al., 2013) in-house validation none
QL-CON-35S-F/nptII-R construct-specific
23-18-17, 23-198, 31807, 31808, 5345, 8338, ATBT04-06, ATBT04-27, ATBT04-30, ATBT04-31, ATBT04-36, BT10 potato, BT12 potato, BT16 potato, BT17 potato, BT18 potato, BT23 potato, BT6 potato, BXN10211, CZW-3, MON1076, MON1445, MON15985, MON1698, MON531, MON757, MON863, MON87460, SPBT02-7, ZW20
Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the nptII gene (Reiting, 2010) in-house validation none
QL-CON-35S-5' primer A/A1-3' primer B construct-specific
RL01-15, RL01-17, dfr GM petunia
Qualitative conventional PCR method for detection of junction between the 35S promoter (P-35S CaMV) and the maize A1 gene (dihydroflavonol-4-reductase) of Zea mays (Meyer et al., 1993) unknown none
QL-ELE-AINT 2-5'/AINT 2-3' element-specific
I-actin
Qualitative real-time PCR (TaqMan) method for detection of rice actin 1 intron (I-ractI) (Mano et al. 2009). in-house validation none
QL-ELE-At-nop-f2/At-nop-r2 element-specific
CS-nos-RHIRD
Qualitative real-time PCR (TaqMan) method for detection of the nopaline synthase gene (nos) from Agrobacterium tumefaciens (BVL G30.40-16) ring trial validation national standard
QL-ELE-bar 2-5'/bar 2-3' element-specific
CS-bar-STRHY
Qualitative real-time PCR (TaqMan) method for detection of the bar gene (Mano et al., 2009) in-house validation none
QL-ELE-B'nase-F-n4/BNaseR-n3 element-specific
CS-barnase
Qualitative real-time PCR (TaqMan) method for detection of the barnase gene (Scholtens et al. 2013) in-house validation none
QL-ELE-BstarF-n2 /BstarR-n2 element-specific
CS-barstar
Qualitative real-time PCR (TaqMan) method for detection of the barstar gene (Xu et al., 2006) in-house validation none
QL-ELE-cry1A 4-5'/cry1A 4-3' element-specific
CS-cry1Ab-BACTU
Qualitative real-time PCR (TaqMan) method for detection of the cry1Ab gene (Scholtens et al. 2013) in-house validation none
QL-ELE-Cry1Ac-F(/R)-n4/Cry1AcR-n2 element-specific
CS-cry1Ac-BACTU
Qualitative real-time PCR (TaqMan) method for detection of the cry1Ac gene (Scholtens et al., 2013) in-house validation none
QL-ELE-cry1A.105 - F1/cry1A.105 - R1 element-specific
CS-cry1A_105-SYNTH
Qualitative real-time PCR (TaqMan) method for detection of the cry1A.105 gene (Dinon et al. 2011) in-house validation none
QL-ELE-Cry1F-F2/Cry1Fr-n2 element-specific
CS-cry1F-BACTU
Qualitative real-time PCR (TaqMan) method for detection of the cry1F gene (Scholtens et al., 2013, with minor modifications in forward primer and probe sequence) in-house validation none
QL-ELE-cry2Ab2 - F/cry2Ab2 - R element-specific
CS-cry2Ab2-BACTU
Qualitative real-time PCR (TaqMan) method for detection of the cry2Ab2 gene (Dinon et al. 2011) in-house validation none
QL-ELE-cry3A-F/cry3A-R element-specific
CS-cry3A-BACTU, CS-mcry3A-SYNTH
Qualitative real-time PCR (TaqMan) method for detection of the cry3A, the modified mcry3A, the cry3A083, the cry3A085 and the chimaera cry3A/cry1Ab gene (Prins et al., 2016) in-house validation national standard
QL-ELE-Cry3Bbf-n2/Cry3Bbr-n2 element-specific
CS-cry3Bb1-BACTU
Qualitative real-time PCR (TaqMan) method for detection of cry3Bb gene (Scholtens et al., 2013; GMDD) in-house validation none
QL-ELE-epsps 1-5'/epsps 3-3' element-specific
CS-CP4epsps-RHIRD
Qualitative real-time PCR (TaqMan) method for detection of the CP4-epsps gene (Scholtens et al. 2013) in-house validation none
QL-ELE-epsps2 1-5'/epsps2 1-3' element-specific
CS-CP4epsps-RHIRD
Qualitative real-time PCR (TaqMan) method for detection of the CP4 epsps gene (Mano et al., 2009) in-house validation none
QL-ELE-npt 1-5'/npt 1-3' element-specific
CS-nptII-ECOLX, V-nptII-ECOLX
Qualitative real-time PCR (TaqMan) method for detection of the nptII gene (Scholtens et al., 2013) in-house validation none
QL-ELE-Patf-n2/Patr-n2 element-specific
CS-pat-STRVR
Qualitative real-time PCR (TaqMan) method for detection of the pat gene (Xu et al., 2006) in-house validation none
QL-ELE-PFMV 1-5'/PFMV 1-3' element-specific
P-FMV
Qualitative real-time PCR (TaqMan) method for detection of P-FMV (Mano et al., 2009) in-house validation none
QL-ELE-P-FMV-F/P-FMV-R element-specific
P-FMV
Qualitative real-time PCR (TaqMan) method for detection of the P-FMV promoter (Debode et al., 2013) in-house validation none
QL-ELE-P-nos-F/P-nos-R element-specific
P-nos-RHIRD
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter of Agrobacterium tumefaciens (Debode et al., 2013) in-house validation none
QL-ELE-P-Rice actin-F/P-Rice actin-R element-specific
P-act1-ORYSA
Qualitative real-time PCR (TaqMan) method for detection of rice actin 1 promoter (P-ractI) (Debode et al. 2013). in-house validation none
QL-ELE-P-SSuAra-F/P-SSuAra-R element-specific
P-rbcS-ARATH
Qualitative real-time PCR (TaqMan) method for detection of SSUAra promoter (ribulose 1.5-biphoshate carboxylase small subunit) of Arabidopsis thaliana (Debode et al., 2013) in-house validation none
QL-ELE-P-TA29-F/P-TA29-R element-specific
P-ta29-TOBAC
Qualitative real-time PCR (TaqMan) method for detection of P-TA29 tapetal specific promoter of Nicotiana tabacum (Debode et al., 2013) in-house validation none
QL-ELE-P-Ubi-F/P-Ubi-R element-specific
P-ubi1-MAIZE
Qualitative real-time PCR (TaqMan) method for detection of ubiquitin promoter of Zea mays (Debode et al., 2013) in-house validation none
QL-ELE-RERIO 2206/RERIO 2207 element-specific
CS-DsRed2 fluorescent protein
Qualitative conventional PCR method for detection of the modified red fluorescent protein (DsRed2) marker gene (Rehbein & Bogerd, 2007) unknown none
QL-ELE-T-g7-F/T-g7-R element-specific
T-transcript 7
Qualitative real-time PCR (TaqMan) method for detection of the g7 terminator from Agrobacterium tumefaciens (Debode et al., 2013) in-house validation none
QL-ELE-T-OCS-F/T-OCS-R element-specific
T-ocs-RHIRD
Qualitative real-time PCR (TaqMan) method for detection of octopine synthase terminator of Agrobacterium tumefaciens (Debode et al., 2013) in-house validation none
QL-ELE-T-35S-F/T-35S-R element-specific
T-35S CaMV
Qualitative real-time PCR (TaqMan) method for detection of the 35S terminator from Cauliflower mosaic virus (Debode et al., 2013) in-house validation none
QL-ELE-Vip3A-F/Vip3A-R element-specific
CS-vip3Aa20-BACTU
Qualitative real-time PCR (TaqMan) method for detection of the vip3A gene (Liang et al., 2013) in-house validation none
QL-ELE-00-001 element-specific
P-35S CaMV
Qualitative conventional PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Official Collection of Methods according to § 64 LFGB, L 00.00-31) ring trial validation national standard
QL-ELE-00-002 element-specific
CS-nptII-ECOLX, V-nptII-ECOLX
Qualitative conventional PCR method for detection of neomycin phosphotransferase II gene (ISO 21569) ring trial validation ISO standard
QL-ELE-00-003 element-specific
CS-nptII-ECOLX, V-nptII-ECOLX
Qualitative conventional PCR method for detection of Neomycin phosphotransferase II gene (L 00.00-31, 2001) ring trial validation national standard
QL-ELE-00-004 element-specific
P-35S CaMV
Qualitative conventional PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Lipp et al., 2001) ring trial validation ISO standard
QL-ELE-00-005 element-specific
P-35S CaMV
Qualitative conventional PCR method for detection of Cauliflower Mosaic Virus 35S promoter (ISO/FDIS 21569) ring trial validation ISO standard
QL-ELE-00-006 element-specific
T-nos-RHIRD
Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31) ring trial validation national standard
QL-ELE-00-007 element-specific
T-nos-RHIRD
Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998) ring trial validation national standard
QL-ELE-00-008 element-specific
P-nos-RHIRD
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter (ISO/TS 21569-4) ring trial validation ISO standard
QL-ELE-00-009 element-specific
T-nos-RHIRD
Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001) ring trial validation ISO standard
QL-ELE-00-010 element-specific
P-FMV
Qualitative conventional PCR method for detection of Figwort mosaic virus 34S promoter (Pan et al., 2007) ring trial validation ISO standard
QL-ELE-00-011 element-specific
T-nos-RHIRD
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1) ring trial validation ISO standard
QL-ELE-00-012 element-specific
E-35S-CaMV, P-35S CaMV, P-scp1-SYNTH
Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S).

Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1)

ring trial validation ISO standard
QL-ELE-00-013 element-specific
T-nos-RHIRD
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos).

Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1)

ring trial validation ISO standard
QL-ELE-00-014 element-specific
CS-bar-STRHY
Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (ISO 21569 AMD1) ring trial validation ISO standard
QL-ELE-00-015 element-specific
E-FMV, P-FMV
Qualitative real-time PCR (TaqMan) method for detection of figwort mosaic virus 34S promoter (ISO/TS 21569-5). ring trial validation ISO standard
QL-ELE-00-016 element-specific
CS-cry1Ab-BACTU, CS-cry1Ab_Ac-SYNTH, CS-cry1Ac-BACTU
Qualitative real-time PCR (TaqMan) method for detection of the element coding for synthetic cry1Ab/Ac genes (ISO/TS 21569-6) ring trial validation ISO standard
QL-ELE-00-017 element-specific
P-35S CaMV
Qualitative real-time PCR (SYBRGreen) method for detection of P-35S CaMV (Barbau-Piednoir et al., 2014) ring trial validation unknown
QL-ELE-00-018 element-specific
T-nos-RHIRD
Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014) ring trial validation unknown
QL-ELE-00-019 element-specific
CS-CP4epsps-RHIRD
Qualitative real-time PCR (SYBRGreen) method for detection of CS-CP4epsps-RHIRD (Barbau-Piednoir et al., 2014) ring trial validation unknown
QL-ELE-00-020 element-specific
CS-cry1Ab-BACTU
Qualitative real-time PCR (SYBRGreen) method for detection of CS-cry1Ab-BACTU (Barbau-Piednoir et al., 2014) ring trial validation unknown
QL-ELE-00-021 element-specific
CS-pat-STRVR
Qualitative real-time PCR (SYBRGreen) method for detection of CS-pat-STRVR (Barbau-Piednoir et al., 2014) ring trial validation unknown
QL-ELE-00-022 element-specific
CS-bar-STRHY
Qualitative real-time PCR (SYBRGreen) method for detection of CS-bar-STRHY (Barbau-Piednoir et al., 2014) ring trial validation unknown
QL-ELE-00-023 element-specific
T-35S CaMV
Qualitative real-time PCR (SYBRGreen) method for detection of T-35S pCAMBIA sequences (EU-RL GMFF GMOMETHODS database as of January 20, 2017) ring trial validation EU reference method
QL-ELE-00-024 element-specific
T-rbcS_E9-PEA
Qualitative real-time PCR (TaqMan) method for detection of the terminator of the pea ribulose-1,5-bisphosphate carboxylase small subunit (rbcS) E9 gene (EU-RL GMFF GMOMETHODS database as of January 20, 2017) ring trial validation EU reference method
QL-ELE-00-025 element-specific
CS-pat-STRVR
Qualitative real-time PCR (TaqMan) method for detection of the synthetic phosphinothricin acetyltransferase (pat) gene (BVL L00.00-154). EU-RL GMFF GMOMETHODS database as of December 23, 2016. ring trial validation EU reference method
QL-EVE-BN-002 event-specific
GT73
Qualitative conventional PCR method for the detection of oilseed rape event GT73 (ISO 21569). ring trial validation ISO standard
QL-EVE-CP-001 event-specific
Huanong No. 1
Qualitative conventional PCR method for the detection of papaya event Huanong No. 1 (EU-RL GMFF GMOMETHODS database as of September 19, 2016) ring trial validation EU reference method
QL-EVE-DC-001 event-specific
123.2.38
Qualitative conventional PCR method for the detection of carnation event 123.2.38 (FLO-40644-6, previously known as FLO-40644-4) (verified by the CRL GMFF in the context of Commission Decision 2007/364/EC)(EURL GMFF GMOMETHODS database as of May 18, 2016). in-house validation EU reference method
QL-EVE-DC-003 event-specific
25958
Qualitative conventional PCR method for the detection of carnation event 25958 (verified by the EURL GMFF in the context of Commission Implementing Decision (EU) 2015/692) (EURL GMFF GMOMETHODS database as of June 1, 2016) in-house validation EU reference method
QL-EVE-DC-004 event-specific
26407
Qualitative PCR method for detection of carnation event 26407 (verified by the EURL GMFF in the context of Commission Implementing Decision (EU) 2015/694) (EURL GMFF GMOMETHODS database as of June 1, 2016) in-house validation EU reference method
QL-EVE-DC-005 event-specific
27531
Qualitative PCR method for detection of carnation event 27531 (verified by the EURL GMFF in the context of Commission Implementing Decision (EU) 2016/2050) (EURL GMFF GMOMETHODS database as of December 1, 2016) in-house validation EU reference method
QL-EVE-DC-006 event-specific
123.8.8
Qualitative conventional PCR method for detection of carnation event 123.8.8 (EURL GMFF GMOMETHODS database as of December 15, 2017). in-house validation EU reference method
QL-EVE-EC-001 event-specific
PT73 E. coli (TM)
Qualitative real-time PCR (TaqMan) method for the detection of event PT73 (TM) (E. coli K-12 event AG3139) (EU-RL GMFF GMOMETHODS database as of November 24, 2017) ring trial validation EU reference method
QL-EVE-EC-002 event-specific
PL73 E. coli (LM)
Qualitative real-time PCR (TaqMan) method for the detection of event PL73 (LM) (E. coli K-12 strain 19E) (EURL GMFF GMOMETHODS database as of November 24, 2017) ring trial validation EU reference method
QL-EVE-MDB110/SHA016 event-specific
OXY-235
Qualitative real-time PCR (TaqMan) method for detection of of oilseed rape event OXY-235 (EURL GMFF, 2016)) in-house validation EU reference method
QL-EVE-MS-J101-F/J101-R event-specific
J101
Qualitative real-time PCR (TaqMan) method for detection of alfalfa event J101 (Guertler et al. 2018) ring trial validation national standard
QL-EVE-MS-J163-F/J163-R event-specific
J163
Qualitative real-time PCR (TaqMan) method for detection of alfalfa event J163 (Guertler et al. 2018) ring trial validation national standard
QL-EVE-MS-KK179-F/KK179-R event-specific
KK179
Qualitative real-time PCR (TaqMan) method for detection of alfalfa event KK179 (Guertler et al. 2018) ring trial validation national standard
QL-EVE-OS-001 event-specific
LLRICE601
Qualitative real-time PCR (TaqMan) method for detection of rice event LLRICE601 (verified by the EU-RL GMFF in the context of Commission Decision 2006/578/EC). in-house validation none
QL-EVE-Oxy-3F/Oxy-4R event-specific
OXY-235
Qualitative real-time PCR (TaqMan) method for detection of of oilseed rape event OXY-235 (Yang et al. 2008) in-house validation none
QL-EVE-SQ0718/SQ0719 event-specific
MON71800
Qualitative real-time PCR (TaqMan) method for detection of wheat event MON71800 (verification report of the EU-RL GMFF, 4 Dec 2013) in-house validation none
QL-EVE-TA-MON71200 event-specific
MON71200
Qualitative real-time PCR (TaqMan) method for detection of wheat event MON71200 (EU-RL GMFF GMOMETHODS database as of July 3, 2018). in-house validation EU reference method
QL-EVE-ZM-001 event-specific
MON810
Qualitative conventional PCR method for the detection of maize event MON810 (ISO/FDIS 21569). ring trial validation ISO standard
QL-EVE-ZM-002 event-specific
Bt10 maize
Qualitative conventional PCR method for detection of maize event Bt10 (verified by the EU-RL GMFF in the context of Commission Decision 317/2005/EC) in-house validation none
QL-EVE-16-0-1RB-F/16-0-1RB-R event-specific
16-0-1
Qualitative real-time PCR (TaqMan) method for detection of papaya event 16-0-1 (Prins at al., 2016) unknown none
QL-EVE-18-2-4RB-F/18-2-4RB-R event-specific
18-2-4
Qualitative real-time PCR (TaqMan) method for detection of papaya event 18-2-4 (Prins et al., 2016) unknown none
QL-EVE-319FalF/320FalR event-specific
Falcon GS 40/90
Qualitative real-time PCR (TaqMan) method for detection of oilseed rape event Falcon GS40/90 (integration locus 2 – Falcon) (BVL G30.40-6) ring trial validation national standard
QL-EVE-375LibF/376LibR event-specific
Liberator
Qualitative real-time PCR (TaqMan) method for detection of oilseed rape event Liberator (pHoe6/Ac) (BVL G30.40-6) ring trial validation national standard
QL-EVE-558F/558R event-specific
e871 Bacillus subtilis
Qualitative real-time PCR (TaqMan) method for detection of Bacillus subtilis isolate e871 (Paracchini et al., 2017) unknown none
QL-EVE-816AvaF/817AvaR event-specific
Falcon GS 40/90
Qualitative real-time PCR (TaqMan) method for detection of oilseed rape event Falcon GS40/90 (integration locus 1 – Avalon) (BVL G30.40-6) ring trial validation national standard
QL-PLN-00-007 taxon-specific
all-plants
Qualitative conventional PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569) ring trial validation ISO standard
QL-TAX-Acc1-F/Acc1-R taxon-specific
Medicago sativa
Quanlitative real-time PCR (TaqMan) method for detection of alfalfa (Guertler et al. 2018) ring trial validation national standard
QL-TAX-BN-003 taxon-specific
Brassica napus
Qualitative real-time PCR (SYBRGreen) method for detection of oilseed rape cruciferin A gene(Barbau-Piednoir et al., 2014) ring trial validation unknown
QL-TAX-BN-004 taxon-specific
Brassica napus
Qualitative conventional PCR method for detection of oilseed rape high mobility group protein I/Y gene (Pan et al., 2007). ring trial validation unknown
QL-TAX-CaMVF/CaMVR taxon-specific
Cauliflower mosaic virus
Qualitative real-time PCR (TaqMan) method for detection of ORF IV of the coat protein of CaMV virus (Cancar et al., 2005) in-house validation none
QL-TAX-CaMV-ORFV-fd2/CaMV-ORFV-rd2 taxon-specific
Cauliflower mosaic virus
Qualitative real-time PCR (TaqMan) method for detection of the open reading frame V (ORF V) from Cauliflower mosaic virus (CaMV)(BVL G30.40-17) ring trial validation national standard
QL-TAX-CP-001 taxon-specific
Carica papaya
Qualitative conventional PCR method for detection of the chymopapain (chy) gene from papaya (EU-RL GMFF GMOMETHODS database as of May 23, 2016) ring trial validation EU reference method
QL-TAX-DC-001 taxon-specific
Dianthus caryophyllus
Qualitative conventional PCR method for detection of carnation anthocyanidin synthase (EURL GMFF GMOMETHODS database as of June 1, 2016). in-house validation EU reference method
QL-TAX-FMVorf7-F4/FMVorf7-R4 taxon-specific
Figwort mosaic virus
Qualitative real-time PCR (TaqMan) method for detection of orf7 from figwort mosaic virus (Moor et al., 2012) in-house validation national standard
QL-TAX-GM-001 taxon-specific
Glycine max
Qualitative real-time PCR (SYBRGreen) method for detection of soybean lectin gene (Barbau-Piednoir et al., 2014) ring trial validation unknown
QL-TAX-GM-002 taxon-specific
Glycine max
Qualitative conventional PCR method for detection of soybean lectin gene (ISO/FDIS 21569). ring trial validation ISO standard
QL-TAX-GM-008 taxon-specific
Glycine max
Qualitative conventional PCR method for detection of soybean lectin gene (ISO 21569) ring trial validation ISO standard
QL-TAX-OS-002 taxon-specific
Oryza sativa
Qualitative real-time PCR (TaqMan) method for detection of rice Phospholipase D alpha 2 gene (Mazzara et al., 2006). in-house validation unknown
QL-TAX-OS-003 taxon-specific
Oryza sativa
Qualitative real-time PCR (TaqMan) method for detection of rice root-specific GOS9 gene (Hernandez et al., 2005) ring trial validation national standard
QL-TAX-OS-006 taxon-specific
Oryza sativa
Qualitative conventional PCR method for detection of rice sucrose-phosphate synthase gene (Jiang et al., 2009). ring trial validation unknown
QL-TAX-PS-001 taxon-specific
Pisum sativum
Qualitative real-time PCR (TaqMan) method for detection of the pea lectin gene (EU-RL GMFF as of January 23, 2017) ring trial validation EU reference method
QL-TAX-SL-001 taxon-specific
Solanum lycopersicum
Qualitative conventional PCR method for detection of tomato polygalacturonase gene (ISO/FDIS 21569:2005). ring trial validation ISO standard
QL-TAX-SL-005 taxon-specific
Solanum lycopersicum
Qualitative conventional PCR method for detection of tomato LAT52 gene (Yang et al., 2008). ring trial validation unknown
QL-TAX-ZM-002 taxon-specific
Zea mays
Qualitative real-time PCR (SYBRGreen) method for detection of maize alcoholdeydrogenase 1 gene (Barbau-Piednoir et al., 2014) ring trial validation unknown
QL-TAX-ZM-003 taxon-specific
Zea mays
Qualitative conventional PCR method for detection of the maize invertase gene (ISO 21569) ring trial validation ISO standard
QT-CON-00-001 construct-specific
GTS 40-3-2
Quantitative real-time PCR (TaqMan) method for detection of soybean event GTS-40-3-2 (ISO 21570) ring trial validation ISO standard
QT-CON-00-002 construct-specific
GTS 40-3-2
Quantitative real-time PCR (TaqMan) method for detection of soybean event GTS-40-3-2 (Hird et al., 2003). ring trial validation national standard
QT-CON-00-003 construct-specific
GTS 40-3-2
Quantitative real-time PCR (TaqMan) method for detection of soybean event GTS-40-3-2 (ISO/FDIS 21570). ring trial validation ISO standard
QT-CON-00-004 construct-specific
MON80100, MON802, MON809, MON810
Quantitative real-time PCR (TaqMan) method for detection of maize event MON810 (Shindo et al., 2002) ring trial validation ISO standard
QT-CON-00-005 construct-specific
32316, 40416, 4114, 676, 678, 680, A2704-12, A2704-21, A5547-127, A5547-35, DAS1507, DAS59122, DAS59132, Falcon GS 40/90, GU262, HCN10, HCR-1, Liberator, T120-7, T14, T25, T45, Topas 19/2
Quantitative real-time PCR (TaqMan) method for detection of maize event T25 (Shindo et al., 2002) ring trial validation ISO standard
QT-CON-00-006 construct-specific
Bt10 maize, Bt11
Quantitative real-time PCR (TaqMan) method for detection of maize event Bt11 (Shindo et al., 2002) ring trial validation ISO standard
QT-CON-00-007 construct-specific
Bt176
Quantitative real-time PCR (TaqMan) method for detection of maize event Bt176 (Shindo et al., 2002) ring trial validation ISO standard
QT-CON-00-008 construct-specific
GA21
Quantitative real-time PCR (TaqMan) method for detection of maize event GA21 (Shindo et al., 2002) ring trial validation ISO standard
QT-ELE-NOS ter 2-5' /NOS ter 2-3' element-specific
T-nos-RHIRD
Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009) ring trial validation none
QT-ELE-P35S 1-5'/P35S 1-3' element-specific
P-35S CaMV
Quantitative real-time PCR (TaqMan) method for detection of the Cauliflower Mosaic Virus 35S promoter (Kodama et al., 2009) ring trial validation national standard
QT-ELE-00-001 element-specific
P-35S CaMV
Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (Feinberg et al., 2005) ring trial validation unknown
QT-ELE-00-002 element-specific
CS-pat-STRVR
Quantitative real-time PCR (TaqMan)method for detection of phosphinothricin N-acetyltransferase gene (Weighard et al., 2004) ring trial validation unknown
QT-ELE-00-003 element-specific
CS-cry1Ab-BACTU
Quantitative real-time PCR (TaqMan) method for detection of synthetic cryIA(b) gene (ISO/FDIS 21570) ring trial validation ISO standard
QT-ELE-00-004 element-specific
E-35S-CaMV, P-35S CaMV
Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1) ring trial validation ISO standard
QT-EVE-BN-001 event-specific
T45
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event T45 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-BN-002 event-specific
Ms8
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Ms8 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-BN-003 event-specific
Rf3
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Rf3 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-BN-004 event-specific
GT73
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event GT73 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-BN-005 event-specific
Ms1
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Ms1 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) in-house validation EU reference method
QT-EVE-BN-006 event-specific
Rf1
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Rf1 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) in-house validation EU reference method
QT-EVE-BN-007 event-specific
Rf2
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Rf2 (verified by the EU-RL GMFF in the context of Commission Decision 2007/306/EC) in-house validation unknown
QT-EVE-BN-008 event-specific
Topas 19/2
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Topas 19/2 (EU-RL GMFF GMOMETHODS database as of July 21, 2014) in-house validation EU reference method
QT-EVE-BN-009 event-specific
73496
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event 73496 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-BN-010 event-specific
MON88302
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event MON88302 (EU-RL GMFF GMOMETHODS database as of January 27, 2014) ring trial validation EU reference method
QT-EVE-BN-011 event-specific
Ms11
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Ms11 (EU-RL GMFF GMOMETHODS database as of February 15, 2019). ring trial validation EU reference method
QT-EVE-BV-001 event-specific
H7-1
Quantitative real-time PCR (TaqMan) method for detection of sugar beet event H7-1 (EU-RL GMFF GMOMETHODS database as March 13, 2014) ring trial validation EU reference method
QT-EVE-CP-001 event-specific
Huanong No. 1
Quantitative real-time (TaqMan) PCR method for detection of papaya event Huanong No. 1 (EU-RL GMFF GMOMETHODS database as of September 13, 2016) ring trial validation EU reference method
QT-EVE-GH-001a event-specific
281-24-236
Quantitative real-time PCR (TaqMan) method for detection of cotton event 281-24-236 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-GH-001b event-specific
3006-210-23
Quantitative real-time PCR (TaqMan) method for detection of cotton event 3006-210-23 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-GH-002 event-specific
LLCotton25
Quantitative real-time PCR (TaqMan) method for detection of cotton event LLCotton25 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) ring trial validation EU reference method
QT-EVE-GH-003 event-specific
MON1445
Quantitative real-time PCR (TaqMan) method for detection of cotton event MON1445 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) ring trial validation EU reference method
QT-EVE-GH-004 event-specific
MON531
Quantitative real-time PCR (TaqMan) method for detection of cotton event MON531 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) ring trial validation EU reference method
QT-EVE-GH-005 event-specific
MON15985
Quantitative real-time PCR (TaqMan) method for the detection of cotton event MON15985 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) ring trial validation EU reference method
QT-EVE-GH-006 event-specific
GHB614
Quantitative real-time PCR (TaqMan) method for detection of cotton event GHB614 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) ring trial validation EU reference method
QT-EVE-GH-007 event-specific
MON88913
Quantitative real-time PCR (TaqMan) method for detection of cotton event MON88913 (EU-RL GMFF GMOMETHODS database as January 13, 2014) ring trial validation EU reference method
QT-EVE-GH-008 event-specific
GHB119
Quantitative real-time PCR (TaqMan) method for detection of cotton event GHB119 (EU-RL GMFF GMOMETHODS database as of July 21, 2014) ring trial validation EU reference method
QT-EVE-GH-009 event-specific
T304-40
Quantitative real-time PCR (TaqMan) method for detection of cotton event T304-40 (EU-RL GMFF GMOMETHODS database as of January 27, 2014) ring trial validation EU reference method
QT-EVE-GH-010 event-specific
MON88701
Quantitative PCR method for detection of cotton event MON88701 (EURL GMFF GMOMETHODS database as of April 21, 2017) ring trial validation EU reference method
QT-EVE-GM-001 event-specific
FG72
Quantitative real-time PCR (TaqMan) method for detection of soybean event FG72 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-GM-002 event-specific
MON87769
Quantitative real-time PCR (TaqMan) method for detection of soybean event MON87769 (EU-RL GMFF GMOMETHODS database as of July 21, 2014) ring trial validation EU reference method
QT-EVE-GM-003 event-specific
MON87705
Quantitative real-time PCR (TaqMan) method for detection of soybean event MON87705 (EU-RL GMFF GMOMETHODS database as of July 21, 2014) ring trial validation EU reference method
QT-EVE-GM-004 event-specific
A2704-12
Quantitative real-time PCR (TaqMan) method for detection of soybean event A2704-12 (EU-RL GMFF GMOMETHODS database as of October 02, 2014) ring trial validation EU reference method
QT-EVE-GM-005 event-specific
GTS 40-3-2
Quantitative real-time PCR (TaqMan) method for detection of soybean event GTS 40-3-2 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-GM-006 event-specific
MON89788
Quantitative real-time PCR (TaqMan) method for detection of soybean event MON89788 (EU-RL GMFF GMOMETHODS database as of January 23, 2014) ring trial validation EU reference method
QT-EVE-GM-007 event-specific
A5547-127
Quantitative real-time PCR (TaqMan) method for detection of soybean event A5547-127 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-GM-008 event-specific
DP305423
Quantitative real-time PCR (TaqMan) method for detection of soybean event DP305423 (EU-RL GMFF GMOMETHODS database as of January 23, 2014) ring trial validation EU reference method
QT-EVE-GM-009 event-specific
DP356043
Quantitative real-time PCR (TaqMan) method for detection of soybean event DP356043 (EU-RL GMFF GMOMETHODS database as of January 23, 2014) ring trial validation EU reference method
QT-EVE-GM-010 event-specific
MON87701
Quantitative real-time PCR (TaqMan) method for detection of soybean event MON87701 (EU-RL GMFF GMOMETHODS database as of January 23, 2014) ring trial validation EU reference method
QT-EVE-GM-011 event-specific
CV127
Quantitative real-time PCR (TaqMan) method for detection of soybean event CV127 (EU-RL GMFF GMOMETHODS database as of January 23, 2014) ring trial validation EU reference method
QT-EVE-GM-012 event-specific
MON87708
Quantitative real-time PCR (TaqMan) method for detection of soybean event MON87708 (EU-RL GMFF GMOMETHODS database as of January 27, 2014) ring trial validation EU reference method
QT-EVE-GM-013 event-specific
DAS68416
Quantitative real-time PCR (TaqMan) method for the detection of soybean event DAS68416 (EU-RL GMFF GMOMETHODS database as of July 2, 2015) ring trial validation EU reference method
QT-EVE-GM-014 event-specific
DAS81419
Quantitative PCR method for detection of soybean event DAS-81419-2 (EURL GMFF GMOMETHODS database as of May 28, 2015) ring trial validation EU reference method
QT-EVE-GM-015 event-specific
DAS44406
Quantitative PCR method for detection of soybean event DAS-44406-6 (EURL GMFF GMOMETHODS database as of May 28, 2015) ring trial validation EU reference method
QT-EVE-GM-016 event-specific
MON87751
Quantitative PCR method for detection of soybean event MON87751 (EURL GMFF GMOMETHODS database as of April 21, 2017) ring trial validation EU reference method
QT-EVE-GM-017 event-specific
SYHT0H2
Quantitative real-time PCR (TaqMan) method for detection of soybean event SYHT0H2(EU-RL GMFF GMOMETHODS database as of September 19, 2016) ring trial validation EU reference method
QT-EVE-HF-1F/HF-2R event-specific
Huafan No 1
Quantitative real-time PCR (SYBRGreen) method for detection of tomato event Huafan No 1 (Yang et al., 2013) in-house validation unknown
QT-EVE-OS-G6H1-F/G6H1-R event-specific
G6H1
Quantitative real-time PCR (TaqMan) method for detection of rice event G6H1 ring trial validation
QT-EVE-OS-001 event-specific
Golden Rice 2
Quantitative real-time PCR (TaqMan) method for detection of rice event Golden Rice 2 (EU-RL GMFF GMOMETHODS database as of April 19, 2016) ring trial validation EU reference method
QT-EVE-OS-002 event-specific
LLRICE62
Quantitative real-time PCR (TaqMan) method for detection of rice event LLRICE62 (EU-RL GMFF GMOMETHODS database as of January 01, 2014) ring trial validation EU reference method
QT-EVE-ST-001 event-specific
EH92-527-1
Quantitative real-time PCR (TaqMan) method for detection of potato event EH92-527-1 (EU-RL GMFF GMOMETHODS database as January 13, 2014) ring trial validation EU reference method
QT-EVE-ZM-001 event-specific
VCO1981
Quantitative real-time PCR (TaqMan) method for detection of maize event VCO-01981-5 (EURL GMFF GMOMETHODS database as of July 14, 2016) ring trial validation EU reference method
QT-EVE-ZM-002 event-specific
5307
Quantitative real-time PCR (TaqMan) method for detection of maize event 5307 (EURL GMFF GMOMETHODS database as of 2014). ring trial validation EU reference method
QT-EVE-ZM-003 event-specific
MON87427
Quantitative PCR method for detection of maize event MON87427 (EU-RL GMFF GMOMETHODS database as of April 21, 2017). ring trial validation EU reference method
QT-EVE-ZM-004 event-specific
DAS40278
Quantitative real-time PCR (TaqMan) method for maize event DAS40278 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-ZM-005 event-specific
MON87460
Quantitative real-time PCR (TaqMan) method for detection of maize event MON87460 (EU-RL GMFF GMOMETHODS database as of July 21, 2014) ring trial validation EU reference method
QT-EVE-ZM-006 event-specific
Bt11
Quantitative real-time PCR (TaqMan) method for detection of maize event Bt11 (ISO 21570) ring trial validation ISO standard
QT-EVE-ZM-007 event-specific
GA21
Quantitative real-time PCR (TaqMan) method for detection of maize event GA21 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-ZM-008 event-specific
NK603
Quantitative real-time PCR (TaqMan) method for detection of maize event NK603 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-ZM-009 event-specific
MON863
Quantitative real-time PCR (TaqMan) method for detection of maize event MON863 (EU-RL GMFF GMOMETHODS database as of March 13, 2014) ring trial validation EU reference method
QT-EVE-ZM-010 event-specific
DAS1507
Quantitative real-time PCR (TaqMan) method for detection of maize event DAS1507 (EU-RL GMFF GMOMETHODS database as of March 17, 2014) ring trial validation EU reference method
QT-EVE-ZM-011 event-specific
T25
Quantitative real-time PCR (TaqMan) method for detection of maize event T25 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-ZM-012 event-specific
DAS59122
Quantitative real-time PCR (TaqMan) method for detection of maize event DAS59122 (EU-RL GMFF GMOMETHODS database as of March 13, 2014) ring trial validation EU reference method
QT-EVE-ZM-013 event-specific
MIR604
Quantitative real-time PCR (TaqMan) method for detection of maize event MIR604 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-ZM-014 event-specific
GA21
Quantitative real-time PCR (TaqMan) method for detection of maize event GA21 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-ZM-015 event-specific
Bt11
Quantitative real-time PCR (TaqMan) method for detection of maize event Bt11 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-ZM-016 event-specific
MON88017
Quantitative real-time PCR (TaqMan) method for detection of maize event MON88017 (EU-RL GMFF GMOMETHODS database as of March 13, 2014) ring trial validation EU reference method
QT-EVE-ZM-017 event-specific
LY038
Quantitative real-time PCR (TaqMan) method for detection of maize event LY038 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-ZM-018 event-specific
MON89034
Quantitative real-time PCR (TaqMan) method for detection of maize event MON89034 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) ring trial validation EU reference method
QT-EVE-ZM-019 event-specific
3272
Quantitative real-time PCR (TaqMan) method for detection of maize event 3272 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) ring trial validation EU reference method
QT-EVE-ZM-020 event-specific
MON810
Quantitative real-time PCR (TaqMan) method for detection of maize event MON810 (ISO/FDIS 21570) ring trial validation ISO standard
QT-EVE-ZM-021 event-specific
98140
Quantitative real-time PCR (TaqMan) method for detection of maize event 98140 (EU-RL GMFF GMOMETHODS database as January 10, 2014) ring trial validation EU reference method
QT-EVE-ZM-022 event-specific
MIR162
Quantitative real-time PCR (TaqMan) method for detection of maize event MIR162 (EU-RL GMFF GMOMETHODS database as of January 10, 2014) ring trial validation EU reference method
QT-EVE-ZM-023 event-specific
Bt176
Quantitative real-time PCR (TaqMan) method for detection of maize event Bt176 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) in-house validation EU reference method
QT-EVE-ZM-024 event-specific
MON87411
Quantitative PCR method for detection of maize event MON87411 (EURL GMFF GMOMETHODS database, as of April 21, 2017) ring trial validation EU reference method
QT-EVE-ZM-025 event-specific
MON87403
Quantitative PCR method for detection of maize event MON 87403 (EURL GMFF, GMOMETHODS database, as of May 09, 2018) ring trial validation EU reference method
QT-EVE-ZM-026 event-specific
4114
Quantitative PCR method for detection of maize event 4114 (EURL GMFF GMOMETHODS database, as of May 24, 2018). ring trial validation EU reference method
QT-EVE-ZM-027 event-specific
MZHG0JG
Quantitative real-time PCR (TaqMan) method for detection of MZHG0JG. (EURL GMFF GMOMETHODS database as of 2018). ring trial validation EU reference method
QT-EVE-ZM-028 event-specific
MZIR098
Quantitative real-time PCR (TaqMan) method for detection of MZIR098. (EURL GMFF GMOMETHODS database as of 2018). ring trial validation EU reference method
QT-TAX-BN-001 taxon-specific
Brassica napus
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape acyl-ACP thioesterase (FatA) gene (EU-RL GMFF GMOMETHODS database as of January 23, 2014)

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation EU reference method
QT-TAX-BN-002 taxon-specific
Brassica napus
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin storage protein (EU-RL GMFF GMOMETHODS database as of January 27, 2014).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 4

ring trial validation EU reference method
QT-TAX-BN-012 taxon-specific
Brassica napus
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin A (CruA) gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2

ring trial validation EU reference method
QT-TAX-BV-013 taxon-specific
Beta vulgaris
Quantitative real-time PCR (TaqMan) method for detection of sugar beet glutamine synthetase (GS2) gene (EU-RL GMFF GMOMETHODS database as of March 05, 2014) ring trial validation EU reference method
QT-TAX-CP-001 taxon-specific
Carica papaya
Quantitative real-time (TaqMan) PCR method for detection of papaya chymopapain (chy) gene (EU-RL GMFF GMOMETHODS database as of September 13, 2016) ring trial validation EU reference method
QT-TAX-FatA primer1/FatA primer2 taxon-specific
Brassica napus
Quantitative real-time PCR (TaqMan) method for the detection of oilseed rape (Monsanto, 2004, A recommended procedure for real-time quantitative TaqMan PCR for Roundup Ready canola RT73).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2

in-house validation none
QT-TAX-GH-015 taxon-specific
Gossypium hirsutum
Quantitative real-time PCR (TaqMan) method for detection of cotton fiber-specific acyl carrier protein (ACP1) gene (EU-RL GMFF GMOMETHODS database as of January 07, 2014).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2

ring trial validation EU reference method
QT-TAX-GH-016 taxon-specific
Gossypium hirsutum
Quantitative PCR method for detection of putative cotton SAH7 protein gene (123 bp) (Mazzara et al., 2006).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2

ring trial validation EU reference method
QT-TAX-GH-018 taxon-specific
Gossypium hirsutum
Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of January 17, 2014).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation EU reference method
QT-TAX-GH-019 taxon-specific
Gossypium hirsutum
Quantitative real-time PCR (Taqman) method for detection of cotton alcohol dehydrogenase C gene (EU-RL GMFF GMOMETHODS database as of November 1, 2018).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation EU reference method
QT-TAX-GH-021 taxon-specific
Gossypium hirsutum
Quantitative real-time PCR (TaqMan) method for detection of putative cotton SAH7 protein gene (115bp) (EU-RL GMFF GMOMETHODS database as January 22, 2014).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2

ring trial validation EU reference method
QT-TAX-GM-001 taxon-specific
Glycine max
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene ring trial validation ISO standard
QT-TAX-GM-002 taxon-specific
Glycine max
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (ISO 21570).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation ISO standard
QT-TAX-GM-004 taxon-specific
Glycine max
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (Hird et al., 2003). ring trial validation unknown
QT-TAX-GM-007 taxon-specific
Glycine max
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene ring trial validation ISO standard
QT-TAX-GM-009 taxon-specific
Glycine max
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014) ring trial validation EU reference method
QT-TAX-GM-020 taxon-specific
Glycine max
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation EU reference method
QT-TAX-OS-002 taxon-specific
Oryza sativa
Quantitative real-time PCR (TaqMan) method for detection of rice phospholipase D alpha 2 gene (EU-RL GMFF GMOMETHODS database as of April 19, 2016) ring trial validation EU reference method
QT-TAX-OS-003 taxon-specific
Oryza sativa
Quantitative real-time PCR (TaqMan) method for detection of rice sucrose-phosphate synthase gene (Jiang et al., 2009) ring trial validation unknown
QT-TAX-OS-017 taxon-specific
Oryza sativa
Quantitative real-time PCR (TaqMan) method for the detection of rice phospholipase D alpha 2 (PLD) gene (EU-RL GMFF GMOMETHODS database as of January 13, 2014) ring trial validation EU reference method
QT-TAX-SAD-FW/SAD-RV taxon-specific
Linum usitatissimum
Quantitative real-time PCR (TaqMan) method for detection of flax/linseed (GeneticID NA Inc, 2009; http://gmo-crl.jrc.ec.europa.eu/flax.htm). in-house validation none
QT-TAX-SL-002 taxon-specific
Solanum lycopersicum
Quantitative real-time PCR (TaqMan) method for detection of the tomato LAT52 gene (ISO 21570 Amd1) ring trial validation ISO standard
QT-TAX-SPS primer f/SPS primer r taxon-specific
Oryza sativa
Quantitative real-time PCR (TaqMan) method for detection of the rice sucrose phosphate synthase (SPS) gene (ISO21570 Amd1) ring trial validation ISO standard
QT-TAX-ST-010 taxon-specific
Solanum tuberosum
Quantitative real-time PCR (TaqMan) method for detection of potato UDP-glucose pyrophosphorylase gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014) ring trial validation EU reference method
QT-TAX-wx012-5'/wx012-3' taxon-specific
Triticum aestivum
Quantitative real-time PCR (TaqMan) method for detection of the common wheat waxy-D1 gene (Iida et al., 2005) in-house validation none
QT-TAX-ZM-001 taxon-specific
Zea mays
Quantitative real-time PCR (TaqMan) method for detection of maize alcoholdeydrogenase 1 (adh1) gene.

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation ISO standard
QT-TAX-ZM-002 taxon-specific
Zea mays
Quantitative real-time PCR (TaqMan) method for detection of the maize high-mobility-group A (hmgA) gene (ISO 21570).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation ISO standard
QT-TAX-ZM-003 taxon-specific
Zea mays
Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-TAX-ZM-004 taxon-specific
Zea mays
Quantitative real-time PCR (TaqMan) for detection of maize aldolase 1 (ald1) gene (EU-RL GMFF GMOMETHODS database as of July 14, 2016).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2

ring trial validation EU reference method
QT-TAX-ZM-005 taxon-specific
Zea mays
Quantitative PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of May 28, 2018). ring trial validation EU reference method
QT-TAX-ZM-006 taxon-specific
Zea mays
Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (ISO 21570) ring trial validation ISO standard
QT-TAX-ZM-011 taxon-specific
Zea mays
Quantitative real-time PCR (TaqMan) method for detection of the maize alcohol dehydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014) ring trial validation EU reference method
QT-TAX-ZM-014 taxon-specific
Zea mays
Quantitative real-time PCR (TaqMan) method for detection of maize alcoholdeydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation EU reference method
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