Detection Image Detection methods search result

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QL-BAC-16S-FW/16S-Rev taxon-specific
bacteria
Qualitative conventional PCR for the amplification of the 16S RNA gene from eubacteria and archaebacteria.

This method is meant to enable species identification or assignment of bacterial nucleotide sequence to a taxonomical group of eubacteria and archaebacteria since the target sequence includes variable and highly variable sequences that might serve as signature sequences. Therefore the herein described PCR method should be performed and followed by sequence analysis of the amplified PCR product. (Official Collection of Methods according to § 28b GenTG, G 21.40-1).

ring trial validation national standard
QL-BAC-27f/926R taxon-specific
bacteria
Qualitative conventional PCR for the amplification of the 16S RNA gene from bacteria.

This method is meant to enable species identification or assignment of bacterial nucleotide sequence to a taxonomical group of bacteria since the target sequence includes variable and highly variable sequences that might serve as signature sequences. Therefore the herein described PCR method should be performed and followed by sequence analysis of the amplified PCR product (Official Collection of Methods according to § 28b GenTG, G 21.40-1).

ring trial validation national standard
QL-CON-cptiF2/tNOS-uni-R construct-specific
Kefeng6
Qualitative real-time PCR (TaqMan) method for detection of the junction between the cpti gene and nos terminator (Reiting et al., 2010) in-house validation none
QL-CON-CP1/pFMV2 construct-specific
GT200, GT73, GTSB77, H7-1, J101, J163, MON1445, MON1698, MON87705, MON88302, MON88913, MON89788, RBMT22-082, RBMT22-186, RBMT22-238, RBMT22-262, ZSR500, ZSR502, ZSR503
Qualitative conventional PCR method for detection of the junction between the FMV promoter and the cp4-epsps gene (BVL G30.40-11) ring trial validation national standard
QL-CON-gat/T-pinII-F/gat/T-pinII-R construct-specific
61061, 73496, 98140, DP356043
Qualitative real-time PCR (TaqMan) method for detection of the junction between the gat gene and pinII terminator (Prins et al., 2016) in-house validation national standard
QL-CON-GH-tFreeze-F/GH-tFreeze-R construct-specific
AquAdvantage Salmon
Qualitative real-time PCR (TaqMan) method for detection of the junction between the growth hormone gene and the antifreeze terminator present in AquAdvantage Salmon (Debode et al., 2018) in-house validation none
QL-CON-MDB498 /DPA143 construct-specific
B16 (DLL25), Bt176, CBH351, DBT418, LLCotton25, LLRICE06, LLRICE601, LLRICE604, LLRICE62, MON88701, Ms3, Ms6, T1c-19, T303-3, T304-40, TC6275, W62, W98
Qualitative real-time PCR (TaqMan) method for detection of the junction between the 35S promoter and the bar gene (Bayer CropScience, 2006) in-house validation none
QL-CON-PGS-bar-A2/PGS-bar-B2 construct-specific
Ms1, Ms11, Ms8, RM3-3, RM3-4, RM3-6, Rf1, Rf2, Rf3
Qualitative conventional PCR method for detection of the junction between P-SSU promoter and the bar gene (BVL G30.40-13) ring trial validation national standard
QL-CON-p-nos-F2/NPTII-R construct-specific
1345-4, 16-0-1, 18-2-4, 35 1 N, 55-1, 63-1, B tomato, C5, Da tomato, EH92-527-1, F tomato, F3'5'H GM petunia, FP967, GD743, GFM Cry1A, GS784, HLMT15-15, HLMT15-3, HLMT15-46, Huanong No. 1, INI-FMAPDF, KMD1, MPS965, Melon A, Melon B, Ms1, NF872, RBMT15-101, RBMT21-129, RBMT21-152, RBMT21-350, RL01-15, RL01-17, RM3-3, RM3-4, RM3-6, Rf1, Rf2, SEMT15-02, SEMT15-15, T120-7, Topas 19/2, X17-2, dfr GM petunia
Qualitative real-time PCR (TaqMan) method for detection of the junction between the nopaline synthase promoter (P-nos)and the neomycin phosphotransferase II gene (nptII) (ISO/TS 21569-4) ring trial validation ISO standard
QL-CON-00-001 construct-specific
GTS 40-3-2
Qualitative conventional PCR method for detection of soybean event GTS-40-3-2 (ISO 21569) ring trial validation ISO standard
QL-CON-00-002 construct-specific
Nema282F
Qualitative conventional PCR method for detection of tomato event Nema282F (ISO/FDIS 21569) ring trial validation ISO standard
QL-CON-00-003 construct-specific
Bt10 maize, Bt11
Qualitative conventional PCR method for detection of maize event Bt11 (ISO 21569) ring trial validation ISO standard
QL-CON-00-004 construct-specific
Bt176
Qualitative conventional PCR method for detection of maize event Bt176 (ISO 21569) ring trial validation ISO standard
QL-CON-00-005 construct-specific
32316, 40416, 4114, 678, 680, A2704-12, A2704-21, A5547-127, A5547-35, DAS1507, DAS59122, DAS59132, Falcon GS 40/90, GU262, HCN10, HCR-1, Liberator, T120-7, T14, T25, T45, Topas 19/2
Qualitative conventional PCR method for detection of maize event T25 (ISO 21569) ring trial validation ISO standard
QL-CON-00-006 construct-specific
GTS 40-3-2
Qualitative conventional PCR method for detection of soybean event GTS-40-3-2 (ISO 21569) ring trial validation ISO standard
QL-CON-00-007 construct-specific
Huahui No.1
Qualitative real-time PCR (TaqMan) method for detection of rice event Bt63 (ISO 21569-Amd) ring trial validation ISO standard
QL-CON-00-008 construct-specific
ASR368, GT200, GT73, GTSB77, H7-1, J101, J163, MON1445, MON1698, MON71200, MON71700, MON71800, MON80100, MON802, MON809, MON832, MON87411, MON87427, MON87705, MON88017, MON88302, MON88913, MON89788, NK603, RBMT22-082, RBMT22-186, RBMT22-238, RBMT22-262, ZSR500, ZSR502, ZSR503
Qualitative real-time PCR (TaqMan) method for detection of the junction between the chloroplast transit peptide 2 and the CP4 epsps gene (ISO 21569 AMD1). ring trial validation ISO standard
QL-CON-00-010 construct-specific
FP967
Qualitative real-time PCR (TaqMan) method for detection of a construct present in flax event FP967 (ISO 21569-2) ring trial validation ISO standard
QL-CON-00-011 construct-specific
32316, 40416, 4114, 676, 678, 680, A2704-12, A2704-21, A5547-127, A5547-35, Bt10 maize, Bt11, DAS1507, DAS59122, DAS59132, Falcon GS 40/90, GU262, HCN10, Liberator, MZHG0JG, MZIR098, SYHT0H2, T120-7, T14, T25, T45, Topas 19/2
Qualitative real-time PCR (TaqMan) method for detection of the junction between the 35S promoter and the phosphinothricin N-acetyltransferase (pat) gene (G 30.40-1). ring trial validation national standard
QL-CON-00-012 construct-specific
55-1, 63-1
Qualitative real-time PCR (TaqMan) method for detection of papaya event 55-1 and 63-1 (ISO 21569) ring trial validation ISO standard
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