detail view molecular characterisation GMO molecular characterization details

  • GMO:

    Verdeca HB4
  • Alias:

    Hb4, IND410, 410
  • UID:

    IND-ØØ41Ø-5
  • Tradename:

  • Company:

    Verdeca
  • Developer:

    Verdeca
  • Species:

    Glycine max
    (soybean)
  • Traits:

    • Abiotic stress tolerance > Drought or water tolerance
    • Herbicide tolerance > Glufosinate tolerance

Description:

The event Verdeca HB4 was produced by Agrobacterium-mediated transformation of the soybean variety Williams 82 with the vector pIND2-HB4.

The plants express the homeodomain-leucine zipper 4 gene (hd-4) from sunflower (Helianthus annuus) under the control of its own promoter in response to water availability and abscisic acid. They also contain the bar gene from Streptomyces hygrocopicus encoding the L-phosphinothricin acetyltransferase. Therefore, plants of this event have an increased tolerance to a variety of environmental stress conditions such as drought and salinity of soils and are tolerant to glufosinate-containing herbicides.

A combination of techniques including Southern Blot, DNA sequencing, and bioinformatics confirmed that HB4 soybean contains a single, intact copy of the T-DNA, integrated at a single locus of the soybean genome. A 142 bp sequence of the soybean genome was deleted at the insertion site. HB4 soybean does not contain any vector backbone elements. The stability and integrity of the insert has been shown over six generations.

Sources:

Biotechnology Consultation on Food from GE Plant Varieties submitted under the U.S. Food and Drug Administration (FDA): Biotechnology Consultation Note to the File BNF No. 000155

BCH: Record ID 112020

Links regarding to this GMO:

BCH http://bch.cbd.int/database/record.shtml?documentid=112020, FDA http://www.fda.gov/downloads/Food/IngredientsPackagingLabeling/GEPlants/Submissions/ucm572729.pdf

Number of integration sites: 1

Transformation technique: Agrobacterium-induced

covers integration site(s)

insert only(s)

Name: insert_Verdeca HB4
Comment: Elements according to Biotechnology Consultation File No. 000155. The order of the hd4-cassette and the bar-cassette was not further specified. Sequence information derived from: Early Food Safety Evaluation of HAHB4 protein produced in new plant varieties, submitted to FDA by Verdeca inc., 2015.
Map:
First Reference Sequence:

vector(s)