detail view molecular characterisation GMO molecular characterization details

  • GMO:

    Low PPO5 potato
  • Alias:

    GE potato, gene editing
  • UID:

  • Tradename:

  • Company:

    J.R. Simplot
  • Developer:

    J.R. Simplot
  • Species:

    Solanum tuberosum
    (potato)
  • Traits:

    • Alteration in growth, development or product quality > Reduced black spot bruising

Description:

Low PPO5 potatoes were developed by using disarmed Agrobacterium to transiently deliver transcriptional activator-like effector nuclease (TALEN) into plant cells, generating targeted knockouts through the introduction of a small, targeted mutation in the endogenous ppo5 gene (also known as pot32).

As a result of this small mutation, these potatoes produce reduced PPO5 protein, a polyphenol oxidase (PPO) enzyme that is responsible for enzymatic darkening and discoloration in potato tubers (black spot).

The absence of the binary vector and presence of the desired gene knockout mutation is confirmed by PCR, T7 endonuclease I assay, and Southern blot analysis. No genetic material used to create the knockouts is present in the final potato plants.

Links regarding to this GMO:

USDA APHIS Determination of unregulated status http://www.aphis.usda.gov/biotechnology/downloads/reg_loi/16-320-01_air_response_signed.pdf, Simplot USDA-APHIS inquiry http://www.aphis.usda.gov/biotechnology/downloads/reg_loi/16-320-01_air_inquiry.pdf

Number of integration sites:

Transformation technique: Meganuclease

covers integration site(s)

insert only(s)

Name: edited_Low PPO5 potato
Comment: In event Low PPO5 potato the endogenous Solanum tuberosum ppo5 gene contains a targeted loss-of-function mutation introduced by TALEN. Source Simplot USDA-APHIS inquiry
Map:
First Reference Sequence:
    • Accession:
    • Reference: USDA-APHIS
    • Elements:
      Insert order Name Donor Size (bp) Position
      GE-ppo5-SOLTU Solanum tuberosum -

vector(s)