detail view molecular characterisation GMO molecular characterization details

  • GMO:

    Golden Rice 2
  • Alias:

    GR2, GR2E
  • UID:

    IR-ØØGR2E-5
  • Tradename:

  • Company:

    Syngenta
  • Developer:

    Syngenta
  • Species:

    Oryza sativa
    (rice)
  • Traits:

    • Composition alteration > pro Vitamin A
    • Selection markers and reporter genes > Mannose metabolism

Description:

The event Golden Rice 2 was created by Agrobacterium mediated transformation with the vector pSYN12424 to produce beta (β)-carotene (a form of provitamin A) in the endosperm of the rice grain.

It contains the phytoene synthase (psy) gene from Zea mays, the carotene desaturase I (crtI) gene from Pantoea ananatis, and the phosphomannose isomerase (pmi) gene from Escherichia coli as a selectable marker.

Molecular characterization of the introduced DNA within Golden Rice 2 confirmed the presence at a single insertion site of one copy of the transfer-DNA (T-DNA) region derived from plasmid pSYN12424 that was stably inherited over multiple generations as a single genetic locus according to Mendelian rules of inheritance.

In addition, nucleotide sequencing of the entire inserted DNA, including portions of the 5’ and 3’ flanking rice genomic sequence, confirmed that the T-DNA was inserted without modifications, deletions, or rearrangements, except for small truncations at the 5’ and 3’ termini of 23 bp and 11 bp, respectively.

Sources:

Food Standards Austalia New Zealand (FSANZ) Application A1138

Paine et al. (2005) Improving the nutritional value of Golden Rice through increased pro-vitamin A content. Nature Biotechnology, 23(4), 482-487

Links regarding to this GMO:

FSANZ http://www.foodstandards.gov.au/code/applications/Documents/A1138%20SD1.pdf

Number of integration sites: 1

Transformation technique: Agrobacterium-induced

covers integration site(s)

insert only(s)

Name: insert_Golden Rice 2
Comment: -
Map:
First Reference Sequence:

vector(s)