detail view molecular characterisation GMO molecular characterization details

  • GMO:

    FAD2KO Soybean
  • Alias:

    GE soybean, gene editing, GM026-18, high oleic acid soybean
  • UID:

  • Tradename:

  • Company:

    Calyxt
  • Developer:

    Cellectis plant sciences
  • Species:

    Glycine max
    (soybean)
  • Traits:

    • Composition alteration > Nutritional composition > Altered fatty acids and oils

Description:

FAD2KO Soybean line GM026-18 is a transgene-free knock-out line with mutations in both the FAD2-1A and the FAD2-1B gene.

The fatty acid desaturase 2 (FAD2) determines the level of monounsaturated fats in soybean oil and vegetative tissue. The FAD2KO Soybean knock-out line is homozygous for mutations in both FAD2 genes resulting elevated levels of oleic acid that approximate 80 %.

FAD2KO Soybean line GM026-18 was developed by Agrobacterium rhizogenes-mediated hairy-root transformation of soybean cotyledones of the variety Bert with a TALEN T-DNA expression construct to knock-out FAD2.

The sequence-specific TALENs target all alleles of the FAD2-1A and FAD2-1B genes of soybean. In both genes the target sites are located within 500 bp of the start codon.

The expression constructs consisted of the 35S promoter, two TALEN monomer coding sequences linked by a T2A sequence, and a nos terminator. PCR analyses targeting three distinct regions of the T-DNA construct were used to determine that the TALEN T-DNA construct was segregated away.

Both Haun et al. (2014) Plant Biotech J. 2:934-940 and the Patent WO2014141147A1 describe the line GM026-18. The 2014 “Am I regulated” Inquiry of the company Cellectis for FAD2KO Soybean is less specific with regard to the specific line.

Links regarding to this GMO:

FDA Biotechnology Notification File No. 000164 http://www.fda.gov/downloads/AnimalVeterinary/DevelopmentApprovalProcess/UCM632416.pdf, Patent http://patents.google.com/patent/WO2014141147A1/fi, USDA-APHIS http://www.aphis.usda.gov/biotechnology/downloads/reg_loi/cellectis_air_fad2k0_soy_cbidel.pdf

Number of integration sites:

Transformation technique: Meganuclease

covers integration site(s)

insert only(s)

Name: edited_FAD2KO line GM026-18
Comment: Line GM026-18 contains mutations in both FAD2 genes within the first 500 bp after the start codon: FAD2-1A contains a 63 bp deletion, not leading to a frameshift but removing an essential amino acid domain. FAD2-1B contains a 23 bp frameshift-inducing mutation. The order of the genetic elements is irrelevant.
Map:
First Reference Sequence:

vector(s)