detail view molecular characterisation GMO molecular characterization details

  • GMO:

    Aedes aegypti OX513A
  • Alias:

    LA513A
  • UID:

  • Tradename:

  • Company:

    Oxitec Ltd.
  • Developer:

    Oxitec Ltd.
  • Species:

    Aedes aegypti
  • Traits:

    • Alteration in growth, development or product quality > Reproductive alteration
    • Selection markers and reporter genes > DsRed2 activity

Description:

The Aedes aegypti mosquito line OX513A expresses a conditional lethality trait (in the absence of tetracycline) and a fluorescent marker.

OX513A was produced by microinjecting the #OX513 rDNA construct with a transposase helper plasmid (#265). The rDNA construct consists of regulatory sequences from Ae. aegypti and Drosophila melanogaster, of protein coding sequences from tetracycline transcriptional activator variant known as tTAV (synthetic source), of a DsRed2 gene (from the Discosoma species of marine coral) and of a non‐autonomous transposon inverted terminal repeat sequences (from the Trichoplusiani piggyBac transposable element).

The OX513A line was confirmed to be free of plasmid backbone sequences. The insert has been shown to be stable and a complete single copy insertion.

Links regarding to this GMO:

BCH record 101474 http://bch.cbd.int/database/record.shtml?documentid=101474, FDA-Final environmental Assessment http://www.fda.gov/downloads/AnimalVeterinary/DevelopmentApprovalProcess/GeneticEngineering/GeneticallyEngineeredAnimals/UCM514698.pdf

Number of integration sites: 1

Transformation technique: microinjection

covers integration site(s)

insert only(s)

Name: insert_Aedes aegypti OX513A
Comment:
Map:
First Reference Sequence:

vector(s)