detail view molecular characterisation GMO molecular characterization details

  • GMO:

    AV43-6-G7
  • Alias:

    Modena
  • UID:

    AVE-436G7-1
  • Tradename:

  • Company:

    BASF
  • Developer:

    Avebe
  • Species:

    Solanum tuberosum
    (potato)
  • Traits:

    • Composition alteration > Amylose/amylopectin ratio

Description:

AV43-6-G7 was developed through Agrobacterium-mediated transformation of the potato cultivar Karnico. The intended consequence of the genetic modification in AV43-6-G7 is to inhibit of the expression of granule bound starch synthase protein (GBSS) responsible for amylose biosynthesis. As a result, the starch produced has little or no amylose and consists of amylopectin, which modifies the physical properties of the starch. AV43-6-G7 contains one insert and one copy of an inverted repeat construct of the potato GBSS gene. The target site in the Karnico genome in which the T-DNA had integrated was found to have a deletion of 1814 bp. AV43-6-G7 was confirmed to be free of Agrobacterium-derived vector backbone DNA. Molecular analysis indicated that the DNA insert was stable over several years.

Links regarding to this GMO:

BCH http://bch.cbd.int/database/record.shtml?documentid=101897

Number of integration sites: 1

Transformation technique: Agrobacterium-induced

covers integration site(s)

insert only(s)

Name: insert_AV43-6-G7
Comment: Both border sequences of the T-DNA were truncated during the insertion event. The single insert in AV43-6-G7 consisted of potato DNA for 95 % of all base pairs. The non-potato DNA of the insert was a short sequence originating form a (truncated) lac1 gene of _E. coli_.
Map:
First Reference Sequence:

vector(s)