detail view molecular characterisation GMO molecular characterization details

  • GMO:

    ATBT04-36
  • Alias:

  • UID:

    NMK-89279-1
  • Tradename:

    NewLeaf
  • Company:

    Monsanto Company
  • Developer:

    Monsanto Company
  • Species:

    Solanum tuberosum
    (potato)
  • Traits:

    • Pest resistance > Insect resistance > Coleoptera resistance
    • Selection markers and reporter genes > Kanamycin/neomycin resistance

Description:

ATBT04-36 potato was generated by Agrobacterium tumefaciens-mediated transformation of the male-fertile potato variety Atlantic with the transformation vector PV-STBT04.

ATBT04-36 potatoes constitutively express the cry3A gene from Bacillus thuringiensis ssp. tenebrionis (CS-cry3A-BACTU) which confers a resistance to coleopteran pests and the nptII gene as a selection marker.

Southern Blot analyses indicate that ATBT04-36 potatoes contain three T-DNA copies inserted at three separate loci. One locus consists of the whole plasmid, PV-STBT04. The second copy consists of the T-DNA and the oriV element of the vector backbone. The third insert only contains the cry3A gene.

Links regarding to this GMO:

BCH http://bch.cbd.int/database/record.shtml?documentid=14898, USDA-APHIS http://www.aphis.usda.gov/brs/aphisdocs/95_33801p.pdf

Number of integration sites: 3

Transformation technique: Agrobacterium-induced

covers integration site(s)

insert only(s)

Name: insert2_ATBT04-36
Comment: The whole plasmid was inserted. Source: Amendment to APHIS Petition of the Determinationm of nonregulated Status: Potatoes Producing the Colorado Potato Beetle Control Protein of Bacillus thuringiensis subsp. tenebrionis, Monsanto 1995.
Map:
First Reference Sequence:
Name: insert3_ATBT04-36
Comment: Source: Amendment to APHIS Petition of the Determinationm of nonregulated Status: Potatoes Producing the Colorado Potato Beetle Control Protein of Bacillus thuringiensis subsp. tenebrionis, Monsanto 1995.
Map:
First Reference Sequence:
    • Accession:
    • Reference: USDA-APHIS
    • Elements:
      Insert order Name Donor Size (bp) Position
      1 CS-cry3A-BACTU Bacillus thuringiensis ssp. Tenebrionis -
Name: insert1_ATBT04-36
Comment:
Map:
First Reference Sequence:

vector(s)

Name: vector_PV-STBT04
Comment: Source: Amendment to APHIS Petition of the Determinanion of nonregulated Status: Potatoes Producing the Colorado Potato Beetle Control Protein of Bacillus thuringiensis subsp. tenebrionis. Monsanto (1995)
Map:
First Reference Sequence: