detail view molecular characterisation GMO molecular characterization details

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  • GMO:

    A5547-35

  • Alias:

  • UID:

    ACS-GMØØ8-6

  • Tradename:

    LibertyLink

  • Company:

    AgrEvo

  • Developer:

  • Species:

    Glycine max
    (soybean)

  • Traits:

    • Herbicide tolerance > Glufosinate tolerance

Description:

Southern blot analyses indicated the integration of one intact copy or fragment of the bialaphos resistance gene (CS-pat-STRVR) of transformation vector pB2/35SAcK (vector_ pB2/35SAcK) at a single genomic locus. Using a fragment of the ampicillin resistance gene (V-bla-ECOLX)as a probe in Southern blot analyses suggested its absence in the insert.

Links regarding to this GMO:

BCH http://bch.cbd.int/database/record.shtml?documentid=15418, USDA-APHIS http://www.aphis.usda.gov/brs/aphisdocs/96_06801p.pdf

Number of integration sites: 1

Transformation technique: biolistic method

covers integration site(s)

insert only(s)

Name: insert_A5547-35
Comment:
Map:
First Reference Sequence:
    • Accession:
    • Reference: application documents
    • Elements:
      Insert order Name Donor Size (bp) Position
      1 P-35S-CaMV Cauliflower mosaic virus -
      2 CS-pat-STRVR Streptomyces viridochromogenes -
      3 T-35S-CaMV Cauliflower mosaic virus -

vector(s)

Name: vector_pB2/35SAcK
Comment: plasmid pB2/35SAcK is also known as pWRG5143
Map:
First Reference Sequence:
    • Accession:
    • Reference: USDA-APHIS
    • Elements:
      Insert order Name Donor Size (bp) Position
      1 V-RB-RHIRD Agrobacterium tumefaciens 55 189-243
      2 P-35S-CaMV Cauliflower mosaic virus 543 461-1003
      3 CS-pat-STRVR Streptomyces viridochromogenes 552 1012-1563
      4 T-35S-CaMV Cauliflower mosaic virus 203 1582-1784
      5 V-pUC_oriC-SYNTH Synthetic 551 2253-2803
      6 V-bla-ECOLX Escherichia coli 861 3016-3876