detail view molecular characterisation GMO molecular characterization details

  • GMO:

    A2704-21
  • Alias:

  • UID:

    ACS-GMØØ4-2
  • Tradename:

  • Company:

    AgrEvo, Bayer CropScience
  • Developer:

  • Species:

    Glycine max
    (soybean)
  • Traits:

    • Herbicide tolerance > Glufosinate tolerance

Description:

Southern blot analysis indicated the integration of five intact copies or fragments of CS-pat-STRVR into the host genome. Further analysis also detected vector backbone in the host genome (two fragments of V-bla-ECOLX). Other genetic elements present in the plasmid pB2/35SAcK (see vectorpB2/35SAcK) used for transformation were V-RB-RHIRD and V-pUCoriC-SYNTH.

Links regarding to this GMO:

BCH http://bch.cbd.int/database/record.shtml?documentid=14855, USDA-APHIS http://www.aphis.usda.gov/brs/aphisdocs/96_06801p.pdf

Number of integration sites:

Transformation technique: biolistic method

covers integration site(s)

insert only(s)

Name: insert_A2704-21
Comment:
Map:
First Reference Sequence:
    • Accession:
    • Reference: application documents
    • Elements:
      Insert order Name Donor Size (bp) Position
      1 P-35S-CaMV Cauliflower mosaic virus -
      2 CS-pat-STRVR Streptomyces viridochromogenes -
      3 T-35S-CaMV Cauliflower mosaic virus -
      4 V-bla-ECOLX Escherichia coli -

vector(s)

Name: vector_pB2/35SAcK
Comment: plasmid pB2/35SAcK is also known as pWRG5143
Map:
First Reference Sequence:
    • Accession:
    • Reference: USDA-APHIS
    • Elements:
      Insert order Name Donor Size (bp) Position
      1 V-RB-RHIRD Agrobacterium tumefaciens 55 189-243
      2 P-35S-CaMV Cauliflower mosaic virus 543 461-1003
      3 CS-pat-STRVR Streptomyces viridochromogenes 552 1012-1563
      4 T-35S-CaMV Cauliflower mosaic virus 203 1582-1784
      5 V-pUC_oriC-SYNTH Synthetic 551 2253-2803
      6 V-bla-ECOLX Escherichia coli 861 3016-3876