detail view molecular characterisation GMO molecular characterization details

  • GMO:

    A2704-12
  • Alias:

    LL72
  • UID:

    ACS-GMØØ5-3
  • Tradename:

    LibertyLink
  • Company:

    Bayer CropScience
  • Developer:

    AgrEvo
  • Species:

    Glycine max
    (soybean)
  • Traits:

    • Herbicide tolerance > Glufosinate tolerance

Description:

Southern blot, PCR and sequence analyses indicated integration of two copies of the functional bialaphos resistance gene cassette (P-35S-CaMV > CS-pat-STRVR > T-35S-CaMV) of transformation vector pB2/35SacK in a head-to-tail configuration at a single genomic locus. Between these two cassettes there are two non-functional fragments of a disrupted beta-lactamase gene (BSM-bla-ECOLX). The non-functional fragments are inserted in an inverted orientation to each other and do therefore not constitute a functional gene.

Biotechnology Consultation on Food from GE Plant Varieties submitted under the U.S. Food and Drug Administration (FDA) “Statement of Policy: Foods Derived from New Plant Varieties" (the 1992 policy): Biotechnology Consultation Note to the File BNF No. 000055 http://www.fda.gov/Food/FoodScienceResearch/Biotechnology/Submissions/ucm161170.htm

Links regarding to this GMO:

BCH http://bch.cbd.int/database/record.shtml?documentid=14764, EFSA application document http://registerofquestions.efsa.europa.eu/roqFrontend/questionDocumentsLoader?question=EFSA-Q-2005-174

Number of integration sites: 1

Transformation technique: biolistic method

covers integration site(s)

Name: insert_A2704-12
Comment:
Map:
First Reference Sequence:

insert only(s)

vector(s)

Name: vector_pB2/35SAcK
Comment: plasmid pB2/35SAcK is also known as pWRG5143
Map:
First Reference Sequence:
    • Accession:
    • Reference: USDA-APHIS
    • Elements:
      Insert order Name Donor Size (bp) Position
      1 V-RB-RHIRD Agrobacterium tumefaciens 55 189-243
      2 P-35S-CaMV Cauliflower mosaic virus 543 461-1003
      3 CS-pat-STRVR Streptomyces viridochromogenes 552 1012-1563
      4 T-35S-CaMV Cauliflower mosaic virus 203 1582-1784
      5 V-pUC_oriC-SYNTH Synthetic 551 2253-2803
      6 V-bla-ECOLX Escherichia coli 861 3016-3876